过氧化物酶体增殖因子活化受体δ(PPARδ)在大肠癌中的表达及其意义——附80例临床分析  

Different expression and significance of peroxisome proliferator-activated receptor δ in colorectal cancer

作　　者：钟芸诗[1] 姚礼庆[1] 孙益红[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中国现代医学杂志》2003年第22期93-96,共4页China Journal of Modern Medicine

摘　　要：目的　通过研究PPARδ在不同类型大肠癌中的表达差异 ,明确PPARδ的表达与大肠癌病理学特征的关系。方法　选取 2 0 0 2年 5～ 11月该院普外科收治手术的大肠癌病例 80例 ,以同期普外科收治的行PPH术病例的正常直肠黏膜为对照 ,对它们的冰冻病理切片作免疫组织化学染色后用IMSDNA/彩色图像分析系统作图形分析 ,以阳性细胞面积占视野面积的百分比来表示PPARδ表达的阳性率。结果　入选大肠癌病例和正常对照组相比 ,年龄、性别无统计学差异 :PPARδ在细胞内的定位主要位于细胞核内 ,呈现棕黄色。正常大肠组织中的阳性细胞较少 ,大肠癌组织中的阳性细胞主要是增生或恶变的大肠腺细胞 ;正常大肠组织和大肠癌组织的PPARδ表达阳性率分别为 (5 .35± 3.4 2 ) %和 (30 .5 5± 8.81) % (P <0 .0 1) ,具有极显著的统计学差异 ;在大肠癌标本中 :肿瘤的分化程度、肿瘤的侵犯深度和肿瘤的Dukes分期与PPARδ的表达阳性率有关 (P <0 .0 5 )。病理组织类型、淋巴结转移情况和肝转移情况与PPARδ的表达无关 (P >0 .0 5 )。结论　PPARδ的表达与大肠癌的分化程度、浸润深度和Dukes分期有关 ,与大肠癌的病理组织类型、淋巴结转移情况和肝转移情况无关。Objective:Compare the expression of PPARδ in different colorectal cancer to see the relation between PPARδ expression rate and pathological characteristic of colorectal cancer.Methods:The consecutive colorectal cancer patiems in General Surgery of ZhongShan Hospital between May 2002 and Nov. 2002 are registered, and procedure for prolapse and Haemorrhoids(PPH) patients in the same time are selected as the normal control. Their frost specimen immunochemical dyedare analyzed by IMS DNA figure analysis system, the PPARδ positive rate arerecorded to see the relation between PPARδ expression and pathological characteristic of colorectal cancer. The data is analyzed by SPSS, P<0.05 means statistically difference.Results:A total of 80 colorectal cancer cases and 20 PPH cases(normal control) are enrolled, the age and sex composing rates of these two groups have no statistic difference. 1) PPAR 8 expresses mainly in the cell nucleus, seen brown; 2) The positive rate of PPAR 6 in the control group and colorectal cancer group is 5.35±3.42% and 30.52 8.81 respectively (P<0.01) ; 3) The PPARδ positive rate increases when the differentiation of the colorectal cancer specimen decreases, and they have statistic difference.( P<0.05).The same is seen in the different enroding depth specimen and the different Dukes stage specimen(P<0.05).But in different pathological types, lymph metastasis and liver metastasis specimen, there are no statistic differences.Conclusions: PPARδ expression relates to cancer differentiation, enroding depth and Dukes stages, not to pathological type, lymph metastasis and liver metastasis.

关 键 词：PPARΔ 大肠癌 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]