胶质瘤细胞系对γ射线辐射敏感性实验研究  

Experimental study on sensibility to γ ray irradiation of two glioma cell lines

作　　者：曹锐峰[1] 章翔[1] 曹云新[2] 姬西团[1] 王西玲[1] 梁景文[1] 郭国桢[3] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710033 [2]第四军医大学基础部医学免疫学教研室,陕西西安710033 [3]第四军医大学预防医学系放射医学教研室,陕西西安710033

出　　处：《第四军医大学学报》2003年第22期2051-2055,共5页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (39970 752 )

摘　　要：目的 :探讨γ射线杀伤胶质瘤细胞的剂量 效应关系、时间 效应关系和杀伤作用的机制 ,比较两种不同种属来源的胶质瘤细胞系对γ射线照射的敏感性 .方法 :以胶质瘤细胞系C6和SHG 4 4为实验对象 ,应用60 Co放射源产生的γ射线行单次照射 ,设对照组、4Gy ,8Gy ,1 6Gy ,32Gy和 6 4Gy组 .照射后光镜下观察细胞形态改变并进行细胞凋亡检测 ,流式细胞术测定照射后不同时间和不同剂量照射时瘤细胞坏死、凋亡和存活的比例 .结果 :照射后 2 4h ,32Gy和 6 4Gy组两种细胞均可见多数细胞出现明显的类似凋亡的细胞形态学改变 ,Hoechst33342染色可见细胞核呈致密浓染 .随着照射后观察时间的延长 ,两种细胞的凋亡率逐渐提高 ,6h后各时间点细胞凋亡率均高于对照组 ,差异有非常显著性 (P<0 .0 1 ) .两种细胞在照射后 4 8h凋亡率与 4 8h前各时间点相比明显增高 ,差异有非常显著性 (P <0 .0 1 ) .4 8h后各个时间点的凋亡率与 4 8h相比 ,无显著差异 (P >0 .0 5 ) .随着照射剂量的增加 ,照后 4 8h两种细胞凋亡率均逐渐增高 ,与对照组相比差异有非常显著性 (P <0 .0 1 ) ,但 32Gy以上剂量照射后细胞凋亡率趋于稳定 .在各种照射剂量下两种胶质瘤细胞坏死率与对照组相比均无显著差异 (P >0 .0 5 ) .SHG 4 4细胞与C6细胞相比 ,在吸?AIM: To study the dose and time dependence in the killing effect of γ ray irradiation for two glioma cell lines, investigate the underlying mechanism and compare the radiosensitivity of the two lines. METHODS: Both C6 and SHG 44 cells were divided into 6 groups: 4 Gy, 8 Gy, 16 Gy, 32 Gy, 64 Gy and negative control. A single irradiation was given to achieve each absorbed dose. After irradiation, changes in cell morphology were studied by an invert microscope. To distinguish the possible cell apoptosis, Hoechst 33258 was used to stain the cells nuclei. A flow cytometry was conducted to analyze the composition of the cells by determining the fractions of apoptosis, necrosis and normal in several different time phases after 16 Gy irradiation and also 48 h after the irradiation of each doses. RESULTS: 24 h after 64 Gy or 32 Gy irradiation, many cells of both two lines showed apparent changes in morphology. The classical appearance of cells with apoptosis could be found in the irradiated cells stained by Hoechst 33258. 6 h after irradiation, the apoptosis rates of both cell lines were significantly higher than those of control and they increased greatly with time till 48 h after irradiation. As the absorbed doses raised, the apoptosis rates of both cell lines increased significantly( P <0.01 vs control) but the necrosis rates remained stable( P >0.05 vs control). In 4 Gy, 32 Gy and 64 Gy group, the SHG 44 cells had higher apoptosis rates than C6 cells( P <0.01). CONCLUSION: γ ray irradiation (≤64 Gy) can kill the two glioma cell lines mainly by inducing cell apoptosis. The apoptosis peak appears about 48 h after irradiation. There is a dose dependence in the killing effects of γ ray irradiation. SHG 44 cells are more sensitive to γ ray radiation than C6 cells.

关 键 词：神经胶质瘤 脱噬作用 细胞系 Γ射线 流式细胞术 辐射敏感性 杀伤作用 

分 类 号：R730.264[医药卫生—肿瘤] R730.55[医药卫生—临床医学]