基于汞离子氧化作用的硫胺素液滴光化学传感器研究  

Studies on Droplet Optical Chemistry Sensor of Thiamine Based on It′s Oxidation by Mercury Ion

作　　者：冯锋[1,2] 王柯敏[1] 杨荣华[1] 周雷激[1] 黄红梅[1] 黄杉生[1] 李杜[1] 

机构地区：[1]湖南大学化学化工学院,生物传感与化学计量学国家重点实验室,长沙410082 [2]山西雁北师范学院化学系,大同037000

出　　处：《高等学校化学学报》2003年第12期2189-2191,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重点项目 (批准号 :2 0 13 5 0 10 );国家杰出青年基金 (批准号 :2 982 5 110 );山西省自然科学基金 (批准号 :2 0 0 110 0 4)资助

摘　　要：A novel renewable liquid drop sensor was proposed for quantitative assay of thiamine based on it′s oxidation by Hg 2+ in basic medium. The dynamically growing and falling drops formed at the end of a silanized silica capillary tube served as the reactors for a chromogenic reaction and windowless optical cells as well. The optimum analytical conditions have been established. The sensor showed a linear response in the measuring range from 1.0×10 -4 to 5.0×10 -3 mg/mL thiamine, and with a detection limit of 8.0×10 -4 mg/mL. Besides its high sensitivity, the sensor permits a simple, fast, and inexpensive measurement with only micro-quantities reagent consumption.A novel renewable liquid drop sensor was proposed for quantitative assay of thiamine based on it′s oxidation by Hg 2+ in basic medium. The dynamically growing and falling drops formed at the end of a silanized silica capillary tube served as the reactors for a chromogenic reaction and windowless optical cells as well. The optimum analytical conditions have been established. The sensor showed a linear response in the measuring range from 1.0×10 -4 to 5.0×10 -3 mg/mL thiamine, and with a detection limit of 8.0×10 -4 mg/mL. Besides its high sensitivity, the sensor permits a simple, fast, and inexpensive measurement with only micro-quantities reagent consumption.

关 键 词：汞离子 氧化作用 硫胺素 液滴光化学传感器 荧光分析法 药品检测 维生素 

分 类 号：R977.2[医药卫生—药品] R927.1[医药卫生—药学]