乙型肝炎病毒C基因截短对S基因转录与表达的影响  

The effect on transcription and translation of S gene in the C-truncated HBV mutant

作　　者：韩聚强[1] 胡大荣[1] 李娟[1] 范公忍[1] 胡学玲[1] 刘超英[1] 刘勇[1] 邸雅南[1] 吴忆贫[1] 

机构地区：[1]北京军区总医院肝病研究所,北京100700

出　　处：《中华微生物学和免疫学杂志》2003年第11期849-852,共4页Chinese Journal of Microbiology and Immunology

基　　金：全军"十五"医药卫生基金资助项目 (0 1MA0 10 )

摘　　要：目的　研究HBVC基因截短对S基因转录与表达的影响。方法　采用分子克隆、定点突变技术构建C基因截短型HBV载体 ,瞬时转染HepG2细胞。应用RT PCR、实时荧光定量PCR检测S基因的转录 ;应用Westernblot、ELISA检测S蛋白的表达。结果　经酶切鉴定 ,C基因截短型HBV载体构建成功 ;C基因截短对HBVS基因转录没有影响 ,但C基因截短 14 1nt后可显著提高HBVS蛋白表达。结论　HBVC基因截短可影响HBVS基因的生物学功能 ,机制发生在转录后或翻译水平。Objective To investigate the effect on transcription and translation of HBV S gene after C gene was truncated. Methods The C-truncated HBV vectors were constructed by a molecular clone and PCR based site directed mutagenesis in vitro , and were then transfected. The transcription of S gene was quantitatively tested by RT-PCR and real-time fluorescence RT-PCR assays. The translation of S protein was quantitatively evaluated by Western blot assay and ELISA. Results The HBV vectors with truncated C gene were successfully constructed. There was no difference in S gene transcription between the C-truncated HBV and the wild HBV. However, the translating efficacy of S protein was improved after C gene was truncated in 267-407nt. Conclusion The truncated-C gene has some impacts on the biological function of HBV S gene by the possible post-transcription and translation mechanisms.

关 键 词：乙型肝炎病毒 C基因截短 S基因 转录 表达 翻译 

分 类 号：R392[医药卫生—免疫学]