人端粒酶逆转录酶与bcl-2在成釉细胞瘤中的表达  

Expression of Human Telomerase Reverse Transcriptase and bcl-2 in Ameloblasomas

作　　者：王洁[1] 钟鸣[1] 姜晓宏[2] 王兆元[1] 张波[3] 侯琳[3] 

机构地区：[1]中国医科大学口腔医院病理科,110002 [2]沈阳市口腔医院正畸科 [3]北京大学医学部病理系

出　　处：《华西口腔医学杂志》2003年第6期441-443,共3页West China Journal of Stomatology

基　　金：辽宁省教育厅资助项目 (编号 2 0 1 2 2 1 7)

摘　　要：目的　研究成釉细胞瘤 (AB)中人端粒酶逆转录酶 (hTERT)和bcl_2的表达。方法　采用原位杂交或免疫组化方法对AB(分原发、复发和恶变组 )中hTERTmRNA和bcl_2进行检测 ,并与正常口腔黏膜作对照。结果　hTERTmRNA阳性表达率在AB中为 94 4 % ,正常口腔黏膜中为 14 3% (P <0 0 0 1) ;bcl_2蛋白阳性率在AB中为88 0 % ,正常口腔黏膜中为 4 4 4 % (P <0 0 0 1)。从表达趋势看伴随着AB的复发与恶变 ,hTERTmRNA与bcl_2表达均增强 (P <0 0 5 )。结论　端粒酶和bcl_2可作为评价AB预后的有效指标。端粒酶的活化和bcl_2表达的增强在AB的发生、发展中起着重要作用。Objective To study the expression of telomerase reverse transcripatase (hTERT) and bcl-2 in ameloblastoma (AB).Methods hTERT mRNA in 54 cases of AB (primary AB 31 cases, recurrent AB 17 cases, malignant AB 4 cases) and 7 cases of oral normal mucosa was detectd by in situ hybridization, and bcl-2 by S-P method.Results The expression of hTERT mRNA was negative or weak in normal oral mucosa (14.3%), moderate or strong in AB (94.4%). There was a significant difference in these two groups (P<0.001). The difference between the expressions of hTERT in primary, recurrent and malignant AB was significant (P<0.05). The positive ratio of bcl-2 in AB and normal oral mucosa was respectirehy 88.0%, 44.4%. There was a significant statistical difference in these two groups (P<0.001). hTERT mRNA was stronger in recurred or malignantly transformed AB (P<0.05).Conclusion The expression of hTERT and bcl-2 is stronger in recurred or malignantly transformed AB, and it could be used as an indicator of AB prognosis. Telomerase activity and bcl-2 expression play an important role in genesis and development of AB.

关 键 词：端粒酶逆转录酶 BCL-2 成釉细胞瘤 表达 免疫组化 原位杂交 

分 类 号：R739.8[医药卫生—肿瘤]