肿瘤坏死因子α和白细胞介素2在大鼠皮肤移植中的作用  

Study on Tumor Necrosis Factor-α and Interleukin 2 in Rat Skin Allograft

作　　者：王军琳[1] 金岩[1] 刘晓亮[1] 

机构地区：[1]第四军医大学口腔医学院组织病理教研室,710032

出　　处：《华西口腔医学杂志》2003年第6期471-473,480,共4页West China Journal of Stomatology

基　　金：国家科技部"863"重大专项课题资助项目 (编号0 0 2AA2 0 50 4 1 )

摘　　要：目的　探讨肿瘤坏死因子α(TNF_α)和白细胞介素 2 (IL_2 )在同种异体大鼠皮肤移植后免疫排斥反应中的作用 ,研究组织工程皮肤的组织相容性。方法　取SD大鼠皮肤和实验室培养的组织工程SD大鼠皮肤移植于成年Wistar大鼠 ,移植成功后取不同时间点标本用免疫组织化学方法和Westernblot方法检测TNF_α和IL_2在组织中的表达。结果　同种异体皮肤移植组 ,TNF_α和IL_2有显著表达 ;组织工程皮肤移植后 ,局部组织中TNF_α和IL_2水平显著低于同种异体皮肤移植组。结论　TNF_α和IL_2水平的改变与同种异体皮肤移植后免疫排斥反应的发生密切相关 ,组织工程皮肤只具有很弱的免疫原性 ,不引起显著的排斥反应。Objective To elucidate the histocompatibility of tissue engineered rat skin through studying the effect of TNF-α and IL-2 in immunological rejection after rat skin allograft.Methods Tissue engineered skin that the basic materials were taken from neonatal SD rats was cultured in lab, grafted to adult Wistar rats. The expressions of TNF-α and IL-2 in grafted tissue were detected with immunohistochemical staining and Western blot.Results The expressions of TNF-α and IL-2 were remarkable in skin allograft group, but low in tissue engineered skin group.Conclusion The expressions of TNF-α and IL-2 and the immunological rejection were closely related after skin allograft, but the tissue engineered skin has favorable histocompatibility and doesn′t arose obvious immunological rejections.

关 键 词：肿瘤坏死因子Α 白细胞介素2 大鼠 皮肤移植 作用 组织工程 

分 类 号：R363[医药卫生—病理学]