有机相中α-氰基-3-苯氧基苄醇乙酯的酶促醇解反应  

Lipase-Catalyzed Alcoholysis of α-Cyano-3-phenoxybenzyl Acetate in Organic Media

作　　者：杨立荣[1] 罗积杏[1] 吴坚平[1] 唐世平[1] 

机构地区：[1]浙江大学材料与化工学院生物工程研究所,杭州310027

出　　处：《有机化学》2003年第11期1260-1263,共4页Chinese Journal of Organic Chemistry

摘　　要：研究了有机相中脂肪酶催化α 氰基 3 苯氧基苄醇乙酯的醇解化反应 ,制备α 氰基 3 苯氧基苄醇 .考察了酶、溶剂、醇、醇用量、溶剂水含量以及底物浓度等因素对反应的影响 ,结果表明Novozym43 5脂肪酶催化活性最高 ,经实验确定的最佳条件为 :脱水甲苯为溶剂 ,正辛醇为酰基受体 ,正辛醇∶酯的摩尔比为 1 5∶1,酶量为 8mg/mL时的最佳底物浓度为 10 8 13mmol/L ,在上述条件下反应 3 0h酯的转化率 >96% .The lipase-catalyzed alcoholysis of α-cyano-3-phenoxybenzyl acetate (CPBAc) in organic solvents for the synthesis of α-cyano-3-phenoxybenzyl alcohol (CPBA) was studied. The experimental results indicated that Novozym435 lipase showed the highest catalytic activity for the reaction. 1-Octanol was founed to be the best acyl acceptor. Toluene was the preferred solvent and the optimum molar ratio of 1-octanol to CPBAc was about 1.5∶1. The reaction rate was slowed down with the addition of water to the solvent. The best concentration of substrate was 108.13 mmol/L under 8 mg/mL lipase for the reaction. When the reaction was carried out under the above optimum conditions, more than 96% of conversion was achieved.

关 键 词：有机相 α-氰基-3-苯氧基苄醇乙酯 酶催化 醇解反应 拟除虫菊酯 杀虫剂 中间体 

分 类 号：O621.25[理学—有机化学]