细胞周期素G_1反义硫代脱氧寡核苷酸抑制HL-60细胞在裸鼠体内生长的实验研究  

Effect of cyclin G_1 antisense oligodeoxynucleotides(ASON)on the growth of HL-60 cells in nude mice

作　　者：贾晋松[1] 徐世荣[1] 贾存荣[2] 马劼[1] 哈森[3] 姚银荣[2] 王毅[2] 石翠英[2] 

机构地区：[1]河北医科大学第二医院血液内科,石家庄050000 [2]河北省血液中心 [3]内蒙古医学院附属第一医院血液内科

出　　处：《中华血液学杂志》2003年第12期648-651,共4页Chinese Journal of Hematology

基　　金：河北省科委基金资助项目 ( 0 2 2 7610 4d -13 )

摘　　要：目的　探讨细胞周期素G1 (cyclinG1 )反义硫代脱氧寡核苷酸对HL 6 0细胞在裸鼠体内生长的抑制作用。方法　将裸鼠随机分成对照组、正义寡核苷酸 (SON)组、反义寡核苷酸 (ASON)组 ,其中SON组和ASON组接种转染的HL 6 0细胞 (HL 6 0 S和HL 6 0 AS)前接受 3Gy的6 0 Co照射 ;将各组HL 6 0细胞接种于裸鼠皮下连续观察裸鼠的出瘤时间、瘤体大小 ,计算抑瘤率 ;病理切片用苏木精 伊红染色 ,电镜观察移植瘤组织的结构和细胞形态变化 ;用流式细胞仪 (FCM)和RT PCR分别检测cyclinG1 蛋白和mRNA水平的表达 ;电镜和FCM检测细胞凋亡。结果　①cyclinG1 ASON组与SON组和对照组比较 ,对HL 6 0细胞在裸鼠体内生长有明显抑制作用 ,抑瘤率为 6 9.4 %。成瘤率、瘤重、瘤体积明显小于SON组和对照组。②镜下见cyclinG1 ASON组的HL 6 0细胞部分细胞形态发生改变 ,核分裂象少见 ,细胞的异型性较小 ,并且有凋亡细胞出现。结论　cyclinG1 ASON能明显抑制HL 6 0细胞在裸鼠体内的生长 ,并且促使部分细胞发生凋亡。Objective To study the inhibition effect of cyclin G 1 antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice. Methods ①Nude mice were divided into control group, sense oligodeoxynucleotides (SON) group and ASON group. After 60?Co radiation,with HL-60 cells SON group and ASON group were subcutaneously innoculated;②The weight and volume of tumors were continually measured;③The morphology of tumor cells was observed by microscope;④The protein and mRNA expression levels of cyclin G 1 were determined by flow cytometry (FCM) and reverse transcription polymerase chain reaction (RT-PCR);⑤The cell apoptosis was detected by electron microscopy and FCM. Results ①The inhibition rate of tumor in ASON group was 69.4%. In ASON group,the wight and volume of tumor were significantly lower than those in SON group and control group. ②The HL-60 cells in ASON group showed morphologically smaller nuclei,less mitosis, less heteromorphosis and apoptosis. Conclusion The cyclin G 1 ASON can inhibit the growth of HL-60 cells in nude mice and induce apoptosis.

关 键 词：细胞周期素G1 反义硫代脱氧寡核苷酸 HL-60细胞 裸鼠 体内生长 实验研究 白血病 

分 类 号：R73-3[医药卫生—肿瘤]