猫部分背根切断后背根节一氧化氮合酶的表达变化  

Expressional Change of Nitric Oxide Synthases in Dorsal Root Ganglia of Cats after Selective Dorsal Rhizotomy

作　　者：覃华丽[1] 周雪[1] 章为[1] 陈思秀[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041

出　　处：《四川大学学报（医学版）》2004年第1期29-31,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 980 0 191);四川省科委基金资助

摘　　要：目的　了解一氧化氮合酶 (NOS)在背根损伤的背根节及邻近未受损之背根节中表达的变化。方法　采用部分去背根传入动物模型 ,通过免疫组织化学 ABC法 ,观察猫背根节中 n NOS及 e NOS同工酶阳性免疫产物的分布、阳性神经元的数量及变化。结果　猫背根节中 n NOS阳性反应物主要分布于小神经元中 ;背根被切断的 L5背根节及邻近完好的 L6 备用背根节中 n NOS阳性小神经元数量较非术侧均有增加 ;e NOS在成年猫背根节神经元中未见表达。结论　在背根节神经元中表达的 n NOS及由 n NOS催化产生的 NO可能与神经损伤和修复的生理病理过程有关。Objective To examine the expressional change of nitric oxide synthase(NOS) in the injured dorsal root ganglia(DRG) and the ipsilateral adjacent uninjured DRG after selective dorsal rhizotomy. Methods Immunochemical ABC method was used to detect the distribution of immunoreaction complex of NOS isoforms--nNOS and eNOS, and quantitative analysis was conducted to get the number of nNOS-immunoreactivity(nNOS-IR) neurons in normal DRG, dorsal rhizotomized DRG and spared DRG from adult cats on the 6th day after operation. This operating model was made by rhizotomizing unilateral L 1-L 5 dorsal roots and leaving L 6 as a spared root. Results nNOS-immunoreactants were mainly distributed in the small-sized neurons in the DRG of cat. The percentage of nNOS-expressing small-sized neurons increased in the deafferentated L 5 DRG(29.74%) when compared with the contralateral DRG(19.35%), and it also increased in the spared DRG(24.22%), compared with the contralateral DRG(18.61%). eNOS-IR was not observed in the DRG of adult cats. Conclusion nNOS/NO up-regulated in DRG neurons is involved in a wide variety of biological functions under physiological and lesion-induced pathophysiological conditions in nerve system.

关 键 词：一氧化氮合酶 免疫组织化学 背根切断 背根节 

分 类 号：R363[医药卫生—病理学]