地塞米松诱发先天性腭裂对TGF-β_1、TGF-β_2表达的影响  

Effects of dexamethasone on TGF-β_1 and TGF-β_2 expression during the induction of cleft palate in mice

作　　者：邓末宏[1] 龙星[1] 李宏礼[1] 董红梅[1] 

机构地区：[1]武汉大学口腔医学院颌面外科,430079

出　　处：《实用口腔医学杂志》2003年第6期598-600,共3页Journal of Practical Stomatology

摘　　要：目的 :探讨TGF β1、TGF β2 在先天性腭裂发病过程中的作用。方法 :在GD12 12 以磷酸地塞米松注射液 (50mg/kg)经母鼠腹腔注射 ,对照组则以等量生理盐水代替。分别于GD13 12 、GD14 12 、GD1512 取出胎鼠头部标本 ,作冠状位切片。采用免疫组化方法检测TGF β1、TGF β2 的表达。结果 :地塞米松在GD12 12 作用于孕鼠可以诱发小鼠先天性腭裂 ,并使胚胎腭中TGF β1表达较同期正常组高 ,TGF β2 表达较正常组高 ,腭突上抬后的降低被抑制。结论 :地塞米松诱发先天性腭裂与TGF β1、TGF β2Objective:To investigate the effects of TGF-β 1 and TGF-β 2 on cleft palate formation .Methods:30 pregnant NIH mice in experimental group were injected with dexamethasone (50 mg/kg) intr aperitoneally in GD 12 12 ,30 mice in control group were given the same vo lume of NS.Fetuses were removed out in GD13 12 ,GD14 12 and GD15 12 , heads of the mice were sliced in coronal plane.Expression of TGF-β 1 an d TGF-β 2 in embryonic palate were detected by immunohistochemical methods: Results:Dexamethasone (50 mg/kg) induced cleft palate in NI H embryos,and expression of TGF-β 1 and TGF-β 2 in palate tissues was h igher in the experimental group than that in the control (P<0.05). Conclusion:Dexamethasone can promote the expression of TGF-β 1 and TGF-β 2 during palate development,which may be responsible for the format ion of cleft palate.

关 键 词：地塞米松 先天性腭裂 TGF-β1 TGF-Β2 转化生长因子-Β1 转化生长因子-Β2 

分 类 号：R782.22[医药卫生—口腔医学]