白藜芦醇和抗坏血酸对预防非典型肺炎方剂Ⅰ和Ⅵ所致小鼠外周血液淋巴细胞DNA损伤的保护作用  

Protective effect of resveratrol and ascorbic acid on lymphocyte DNA damage in mice induced by prescriptions Ⅰ and Ⅵ of Chinese materia medica against SARS

作　　者：金顺姬[1] 段链[1] 黄梅[1] 杨静玉[1] 于海[1] 赵余庆[2] 吴春福[1] 

机构地区：[1]沈阳药科大学药理教研室,辽宁沈阳110016 [2]辽宁中医学院新药研究中心,辽宁沈阳110000

出　　处：《中草药》2003年第12期1114-1117,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的　研究白藜芦醇和抗坏血酸对预防非典型肺炎方剂 和 致小鼠外周血液淋巴细胞 DNA损伤的保护作用。方法　采用单细胞凝胶电泳方法研究白藜芦醇和抗坏血酸对预防非典型肺炎方剂 和 致小鼠外周血液淋巴细胞 DNA损伤的保护作用。结果　白藜芦醇 ( 5 0 ,10 0 ,2 0 0 m g/ kg× 3d)和抗坏血酸 ( 5 0 ,10 0 ,2 0 0 mg/ kg×3d)对方剂 和 (临床等效量× 3d)所致的小鼠外周血液淋巴细胞 DNA损伤呈剂量依赖性的保护作用 ( P<0 .0 5 )。结论　白藜芦醇和抗坏血酸能够保护预防非典型肺炎方剂 和 对小鼠外周血液淋巴细胞 DNA的损伤。Object To evaluate the protective effect of resveratrol and ascorbic acid on lymphocyte DNA damage in mice induced by prescriptions Ⅰ and Ⅵ of Chinese materia medica against SARS. Methods Resveratrol, ascorbic acid and two prescriptions against SARS were orally administered to the mice for three days. The effect on DNA damage was measured in mice lymphocytes by means of single cell gel electrophoresis assay (SCGE, or Comet assay). Results Resveratrol (50, 100, 200 mg/kg × 3 d) or ascorbic acid (50, 100, 200 mg/kg × 3 d) could dose dependently restore the DNA damage induced by prescription Ⅰ and Ⅵ (equivalent clinical dose×3 d). Conclusion Resveratrol and ascorbic acid could protect the lymphocyte DNA damage in mice induced by prescriptions Ⅰ and Ⅵ of Chinese materia medica against SARS.

关 键 词：白藜芦醇 抗坏血酸 预防非典型肺炎方剂 DNA损伤 单细胞凝胶电泳 

分 类 号：R285.5[医药卫生—中药学]