角膜碱烧伤房水炎性细胞因子变化的实验研究  

An experimental study of TNF-α and IL-1β in aqueous humor after alkali ocular burns in rabbits

作　　者：黄伟奇[1] 陈毓东[1] 林翔[1] 

机构地区：[1]广东医学院附属医院,湛江市524001

出　　处：《眼外伤职业眼病杂志》2003年第10期654-655,共2页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的　探讨肿瘤坏死因子 α(TNF α)和白细胞介素 1β(IL 1β)在眼前段碱烧伤病理过程中的作用机制。 方法　采用放射免疫法检测兔角膜碱烧伤后房水中不同时间的TNF α和IL 1β的含量。 结果　TNF α和IL 1β含量在角膜碱烧伤后 4小时急剧升高 ,2 4小时达到高峰 ,48～ 72小时呈一较高水平 ,与对照组比较差异均有非常显著性意义 (P <0 0 1)。结论　眼前段碱烧伤的发病机制中TNF α和IL 1β介导的炎症途径参与。Objective The tumor necrosis factor-α(TNF-α) and interleukin-1 β(IL-1β) levels in aqueous humor after alkali burned were studied in rabbit eyes and their effects on mechanism of inflammatory mediators were discussed.Methods The levels of TNF-α and IL-1β in their different metabolic times after alkali burns on rabbit eyes were determined by radioimmunoassay.Results The TNF-α and IL-1 β levels increased at the 4h after alkali burns and reached a peak on 24 h.TNFα and IL-1β maintained higher levels during 48-72 h after alkali burns.Those showed significant differences compared to control (P<0.01).Conclusion The findings indicate that TNF-α and IL-1β pathways of inflammation are involved in the pathogensis of alkali burns.

关 键 词：角膜碱烧伤 房水 炎性细胞因子 实验 肿瘤坏死因子-Α 白细胞介素-1Β 

分 类 号：R779.1[医药卫生—眼科]