三种底物对体外培养的神经干细胞分化和迁移能力的影响  

Effects of three substrata on the differentiation and migration of neural stem cells cultured in vitro

作　　者：郑志竑[1] 林玲[1] 胡建石[1] 林建银[1] 

机构地区：[1]福建医科大学分子医学研究中心,福建福州350004

出　　处：《中国神经科学杂志》2003年第6期370-373,共4页

基　　金：福建省科技计划重大项目 (2 0 0 1Z0 2 0 )资助

摘　　要：目的 :探讨不同底物对体外培养的神经干细胞分化和迁移能力的影响。 方法 :采用多聚鸟氨酸、层黏连蛋白、鼠尾胶原等作为底物 ,观察它们对大鼠神经干细胞分化的诱导和对细胞迁移能力的影响。 结果 :三种底物均能诱导神经干细胞的分化 ,诱导分化的能力为层黏连蛋白 >多聚鸟氨酸 >鼠尾胶原 ,多聚鸟氨酸与层黏连蛋白两者联用具有协同叠加效应 ,而且这些底物也具有介导分化后的神经细胞迁移的能力 ,各种底物对细胞迁移影响力的大小与其诱导分化的能力相似。 结论 :多聚鸟氨酸、层黏连蛋白和胶原不同程度上促进神经干细胞分化和迁移。Objective: To investigate the effects of three substrata on the differentiation and migration of neural stem cells cultured in vitro . Methods: Polyornithine,laminin and collagen were used as substrata to induce the differentiation and migration of neural stem cells. Results: All three substrata could induce the differentiation of neural stem cells. The effect of inducing differentiation was laminin>polyornithine>collagen. Laminin and polyornithine have additive effects on differentiation of stem cells. They also have ability to mediate migration of cells that have differentiated,which was similar to that of inducing differentiation. Conclusion: To various degrees,polyornithine,laminin and collagen promote differentiation and imgration of neural stem cells in culture.

关 键 词：体外培养 神经干细胞 细胞分化 细胞迁移 底物 大鼠 多聚鸟氨酸 层黏连蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]