SR-BI在OxLDL诱导人血单核细胞表达致炎细胞因子mRNA中的作用  

The role of SR-BI in the human peripheral blood monocyte expression of proinflammatory cytokines mRNA induced by oxidized low density lipoprotein

作　　者：雷新军[1] 马爱群[1] 耿涛[1] 白玲[1] 张葳[1] 

机构地区：[1]西安交通大学第一医院心血管内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2003年第6期584-587,607,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：西安交通大学教育部教育振兴行动计划

摘　　要：目的　探讨SR BI在OxLDL刺激人血单核细胞表达致炎细胞因子mRNA中的作用。方法　用原位杂交方法研究OxLDL、SR A的特异性抑制性配体 Fucoidin、17β Estrogen对人血单核细胞SR BImRNA表达的影响及其与MCP 1、PDGF、bFGF、IL 10mRNA表达的关系。结果　OxLDL(10～ 30mg·L-1)能剂量依赖性刺激SR BImRNA表达 (均P <0 .0 0 1) ,Fucoidin(5 0～ 2 5 0mg·L-1)能拮抗 2 0mg·L-1OxLDL的诱导效应 (均P <0 .0 0 1) ,同MCP 1、PDGF、bFGF、IL 10四种致炎细胞因子mRNA的表达相一致。 17β Estrogen(1× 10 -9mol·L-1～ 1× 10 -5mol·L-1)抑制上述致炎细胞因子mRNA表达 (均P <0 .0 0 1) ,但剂量依赖性提高SR BImRNA表达 (P <0 .0 0 1) ;在 1× 10 -5mol·L-1时 ,OxLDL(10～ 30mg·L-1)能对抗Fucoidin(2 5mg·L-1)而刺激上述致炎细胞因子mRNA表达 (均P <0 .0 0 1)。结论　SRObjective To explore the function of scavenger receptor class B type I(SR-BI)in the human peripheral blood monocyte expression of proinflammatory cytokines mRNA induced by oxidized low density lipoprotein(OxLDL). Methods With the digoxenin-labeled oligonucleotide-probes in situ hybridization, we investigated the effects of OxLDL, fucoidin and 17β-estradiol on the primarily-cultured human peripheral blood monocyte mRNA expression of SR-BI and relationship between its expression and the mRNA expressions of MCP-1, bFGF, PDGF and IL-10. Results The mRNA expression of SR-BI was significantly increased in a dose-dependent manner after incubating with OxLDL (10～30 mg·L -1)(P<0.001) and fucoidin (50～250 mg·L -1) distinctly inhibited OxLDL(20 mg·L -1) from inducing the expression of SR-BImRNA, which was consistent with expression of the above proinflammatory cytokines mRNA(P<0.001). 17β-estradiol(1×10 -9mol·L -1～1×10 -5mol·L -1)inhibited the mRNA expression of above proinflammatory cytokines, but notably increased expression of SR-BImRNA in a dose-dependent manner(P<0.001);in the precondition of 17β-estradiol (1×10 -5mol·L -1), OxLDL(10～30 mg·L -1) significantly increased the mRNA expression of the above proinflammatory cytokines antagonizing the inhibitory effect of fucoidin(25 mg·L -1)in a dose-dependent manner(P<0.001). Conclusion SR-BI may not participate in the process which OxLDL stimulates human peripheral blood monocyte to give expression to proinflammatory cytokines mRNA.

关 键 词：SR-BI OXLDL 人血单核细胞 炎细胞因子 MRNA 单核细胞 脂蛋白 雌激素 

分 类 号：R394[医药卫生—医学遗传学]