薏苡仁药材指纹图谱的研究与应用  

Fingerprint research and application for semen coicis

作　　者：陈勇[1] 陈碧莲[1] 何云珍[1] 祝明[1] 

机构地区：[1]浙江省药品检验所,浙江杭州310004

出　　处：《药学实践杂志》2003年第6期357-359,共3页Journal of Pharmaceutical Practice

摘　　要：目的:建立薏苡仁的指纹图谱分析方法,应用于实际工作中。方法:采用HPLC-ELSD法对薏苡仁中甘油三酯类成分进行指纹分析,DiscoveryC18为色谱柱,流动相为乙腈-二氯甲烷(59:41),流速为每分钟0.5mL,漂移管温度为70℃,氮气流速为每分钟1.2L。结果:指纹图谱中共有7个主要色谱峰,其相对保留时间的重现性RSD均小于0.3％,精密度RSD均小于0.1％,相对峰面积的重现性RSD均小于1.8％,精密度RSD均小于2.0％,不同产地的薏苡仁指纹图谱稳定,重现性好。结论:该指纹图谱方法可靠、简便,可作为薏苡仁质量控制指标。OBJECTIVE: To establish the fingerprint method for semen coicis. METHODS:The Discovery C18column with acetonitrile - dichloromethanol (59 : 41) as the mobile phase was used in HPLC-ELSD. The flow rate was 0. 5mL/min and ELSD tube temperature is 70℃ , The flow rate of Nitrogen is 1. 2L/min. RESULTS: There are 7 mostly chromatogram in the fingerprint of semen coicis, the relative standard deviation ( RSD) of relative retain time for reproducibility was not more than 0.3% , for precision was not more than 0. 1% . the relative standard deviation (RSD) of relative peak area for reproducibility was not more than 1.8%, for precision was not more than 2. 0% , and the fingerprint for semen coicis of different producing area is very stabilization. CONCLUSION: The method is simple, accurate with a good reproducibility and can be used as a quantitative analysis method for semen coicis.

关 键 词：薏苡仁 中药材 指纹图谱 植物药 HPLC-ELSD法 

分 类 号：R282.71[医药卫生—中药学]