重庆地区精神疾病患者Borna病病毒感染的初步报道  

Detection of Borna disease virus P24 RNA from peripheral blood mononuclear cells in psychiatric patients

作　　者：邹德智[1] 谢鹏[1] 徐平[1] 吴伟[2] 蒙华庆[3] 陈大伟 左联[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,400016 [2]江西医学院附属第二医院神经内科 [3]重庆医科大学附属第一医院精神科,400016 [4]辽宁省盘锦市第二人民医院神经内科

出　　处：《中华精神科杂志》2003年第4期228-230,共3页Chinese Journal of Psychiatry

基　　金：国家自然科学基金资助项目 (3 9870 2 5 2)

摘　　要：目的　研究中国人中是否存在Borna病病毒 (BDV)感染 ,以及探讨BDV感染是否与人类精神疾病有关。方法　收集精神疾病患者 80例 (精神分裂症 5 0例 ,情感性精神障碍 30例 )和健康献血者标本 6 0名。每例采血 10ml,分离外周血单个核细胞 ,提取RNA ;用BDV P2 4基因特异性内外引物进行套式逆转录聚合酶链反应 ,扩增产物经琼脂糖凝胶电泳和特异性探针的Southern印迹杂交检测。结果　 5 0例精神分裂症患者中检出BDV P2 4基因片段 5例 ( 10 % ) ,6 0名健康对照者均未检出 ,两者间的差异有显著性 (P <0 0 5 ) ;30例情感性精神障碍患者中检出 1例 ( 3% ) ,与健康对照者的差异无显著性。结论　重庆地区精神疾病患者中存在BDV感染 ;Objective This preliminary study was to explore whether there was an infection of Borna disease virus (BDV) in a Chinese population and whether BDV infection was related to human psychiatric disease Methods The nested RT PCR was used to detected BDV P24 RNA in peripheral blood mononuclear cells (PBMCs) in 80 psychiatric patients (50 with schizophrenia and 30 with mood disorder) and 60 healthy blood donors in Chongqing, China After the PBMCs being isolated from 10 ml blood in each subject, RNA was extracted and amplified with nested RT PCR using the BDV P24 specific primers The PCR products were confirmed by Southern blot Results There was 5 BDV P24 positive in patients with schizophrenia (10%), 1 positive in patients with mood disorders (3%), while none in healthy donors The positive rate was significantly higher in schizophrenic patients than in donors Conclusions The results suggest that there is BDV infection in Chinese, and BDV infection may be related to schizophrenia

关 键 词：精神疾病 精神分裂症 情感性精神障碍 Boma病病毒 感染 

分 类 号：R749[医药卫生—神经病学与精神病学]