基质分解素1在实验性肝纤维化过程中的酶原表达变化  

Stromelysin-1 proenzyme expression in the processe of experimental liver fibrosis.

作　　者：王宇[1] 高毅[1] 蒋晓青[2] 

机构地区：[1]第一军医大学珠江医院普通外科,广州510282 [2]第一军医大学南方医院麻醉科,广州510515

出　　处：《临床肝胆病杂志》2003年第6期356-357,共2页Journal of Clinical Hepatology

基　　金：广东省自然科学基金资助项目 No 960 3 62

摘　　要：研究实验性肝纤维化过程中基质分解素 - 1(MMP - 3)酶原蛋白表达的动态变化。建立四氯化碳中毒性大鼠肝纤维化模型 ,采用半定量Western免疫印迹方法 ,对肝纤维化不同阶段肝内MMP - 3的酶原蛋白的表达水平进行了检测。在正常大鼠肝组织中有MMP - 3的酶原表达。在肝纤维化发生发展过程中MMP - 3酶原蛋白表达水平逐渐增强 ,至肝纤维化中期到达最高峰 ,到肝纤维化晚期及肝硬化阶段其表达较中期下降 ,但仍高于正常对照水平。MMP - 3在肝纤维化的发生。To investigate the dynamic alteration of stromelysin-1 (metalloproteinase-3,MMP-3) proenzyme protein expression in the processes of experimental liver fibrosis. The carbon tetrachloride-induced hepatic fibrosis rat models were used. MMP-3 proenzyme protein expression in the different stages of liver fibrosis was measured by half quantitative analysis of Western blot. MMP-3 proenzyme protein expression could be detected in normal rat liver. In the pathogenesis and development of liver fibrosis, MMP-3 proenzyme protein expression level increased gradually, with the expression rate being maximum at an intermediate stage of fibrosis. And it decreased gradually during the late liver fibrosis and at thestage of liver cirrhosis, but remained relatively high levels above normal. MMP-3 might participate in pathogenesis of liver fibrosis and cirrhosis as an important role.

关 键 词：基质分解素1 实验性肝纤维化 酶原表达 Western免疫印迹方法 

分 类 号：R575.2[医药卫生—消化系统]