马立克氏病病毒pp38基因启动子和增强子的部分功能  

Promoter-enhancer′s Partial Function of the pp38 Gene of (Marek′s) Disease Virus

作　　者：丁家波[1] 姜世金[1] 孙淑红[1] 王增福[1] 张纪元[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2004年第1期6-8,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 5 44 )

摘　　要：通过 PCR技术 ,以马立克氏病病毒 (MDV) RB1B株基因组 DNA为模板 ,扩增了包括 pp38基因及其启动子 -增强子和终止子在内的 2 2 0 0 bp的核酸片段。将该片段用 Sac 和 Sph 酶定向克隆到 p U C18质粒中 ,构建成重组质粒 ;将重组质粒转染鸡胚成纤维细胞 (CEF) ,用小鼠抗大肠杆菌表达的 pp38血清作间接免疫荧光试验 ,验证 pp38基因的表达。结果 ,在转染的细胞浆中看到了绿色的荧光 。The (38 000) phosphoprotein gene of Marek′s disease virus RB1B strain with its promoter-enhancer and terminer were amplified through polymerase chain reaction(PCR).The (2 200) bp PCR product was cloned into pUC18 vector via restriction-enzyme SacⅠ and SphⅠ.The recombinant plasmid was transfected into chicken embryo fibroblast(CEF) by using liposome.Indirect fluorescent assay(IFA) with mouse-anti pp38 expressed in E.coli was used to identify the expression of pp38,and gray fluorescence on the transfected cells can be seen.It indicated that the promoter-enhancer could be used to construct a new eukaryotic expressing vector.

关 键 词：马立克氏病病毒 PP38基因 启动子 增强子 间接免疫荧光试验 

分 类 号：S852.652[农业科学—基础兽医学] Q78[农业科学—兽医学]