2型登革病毒检测基因芯片的研制  

Construction of a Diagnostic cDNA Microarray for Serotype 2 Dengue Virus Detection

作　　者：肖维威[1] 马文丽[1] 王洪敏[1] 黄海[1] 王艳[1] 张宝[1] 郑文岭[2] 

机构地区：[1]第一军医大学分子生物学研究所,中国广东广州510515 [2]广州军区广州总医院肿瘤分子生物学研究所,中国广东广州510010

出　　处：《生命科学研究》2003年第4期305-309,共5页Life Science Research

摘　　要：利用长片断PCR扩增含几乎全长的2型登革病毒cDNA,酶切PCR扩增产物,通过建立2型登革病毒cDNA文库获取芯片探针.用点样仪将探针制备成2型登革病毒检测基因芯片.杂交时采用限制性显示(Re strictionDisplayRD)技术标记样品,ScanArray芯片扫描仪检测杂交信号.杂交结果显示,样品和2型登革病毒基因芯片杂交的敏感性强、特异性高.A fragment covering almost the full-length cDNA of dengue virus serotype 2 was amplified by long-PCR. Restriction endonucleases Sau3A I were utilized to digest the amplified products, thus a cDNA fragment library of dengue virus serotype 2 was constructed. Probes amplified from the cDNA library were designed and immobilized on chips by Pixsys 5500 Arrayer. Restriction Display method was used to label the samples when they were hybridized with the microarray. After hybridization,the microarrays were scanned with ScanArray Lite scanner. Hybridization results showed that there were no signals on the negative and blank controls, while obvious hybridization signals appeared on the spots of the positive controls and specific probes. It demonstrated that the diagnostic cDNA microarray was very sensitive and specific for detection of the serotype 2 dengue virus.

关 键 词：基因芯片 基因诊断 2型登革病毒 检测 

分 类 号：R373.33[医药卫生—病原生物学] R44[医药卫生—基础医学]