刺激性唾液蛋白含量变化及其临床诊断价值  

Variations of Protein Concentration in Saliva Stimulated and Its Effect on Clinical Diagnosis

作　　者：柴京[1] 冯涛[1] 杜德顺[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院口腔科,北京100730

出　　处：《中国医学科学院学报》2003年第6期714-716,共3页Acta Academiae Medicinae Sinicae

摘　　要：目的研究刺激性唾液蛋白含量变化及其影响因素,并考察其临床诊断价值。方法取33例正常人和73例Sjgren综合征SS患者在无刺激状态与酸刺激状态下的唾液,放射免疫法测定β2微球蛋白(β2-mG)浓度,速率散射比浊法测定分泌型IgASIgA浓度,精密pH试纸测定pH值,SPSS软件对数据进行统计学处理。结果SS患者刺激组唾液β2-mG浓度较非刺激组明显降低,差异有显著性(P<0.01);并较正常人刺激组升高,差异亦有显著性(P<0.05)。正常人3%醋酸刺激组和Vc刺激组唾液与非刺激组比较,流率明显上升,pH值、SIgA和β2-mG浓度均明显下降,差异有显著性(分别P<0.01和P<0.05);3%醋酸刺激组和Vc刺激组唾液测取值之间也有显著性差异(P<0.01)。结论刺激性唾液蛋白浓度降低可能因pH值降低导致流率增加所致,也可能与pH值降低有直接关系。SS患者刺激性唾液蛋白测定具有一定诊断意义,只是与非刺激性唾液的诊断标准不同。To study the variations of protein concentration in saliva stimulated and its effect on clinical diagnosis. Methods The saliva from 33 normal controls and 73 patients with Sjgren syndrome(SS)who were stimulated with acid and not were collected. The concentration of beta 2 microglobulin (β2-mG), secretory immunoglobulin A(SIgA), and pH were measured by Radioimmunoassay, Rate Nephelometry and pH Detection Paper, respectively. SPSS 10.0 was used to determine the mean statistical differences among these groups. Results In patients with SS, the concentration of β2-mG in saliva stimulated with Vc was significantly lower compared with that in saliva not stimulated(P < 0.01); In saliva stimulated with Vc, the concentration of β2-mG in patients with SS was higher than that in normal controls(P < 0.05). In normal controls, compared with that in saliva not stimulated, flow rate in saliva stimulated with 3% acetic acid and Vc was significantly higher(P < 0.01)and pH, concentration of β2-mG and SIgA were significantly lower(P < 0.01 and P < 0.05 respectively); there was a significant difference of flow rate, β2-mG, SIgA and pH in saliva between the subjects stimulated with 3% acetic acid and with Vc(P < 0.01). Conclusions The reason for the decrease of protein concentration in saliva stimulated may be the increase of flow rate caused by the decrease of pH or the decrease of pH itself. Protein detection of saliva stimulated in patients with SS is helpful in diagnosis, but the criterion is different between the saliva stimulated and not stimulated.

关 键 词：刺激性唾液 流率 分泌型IGA Β2-微球蛋白 SJOEGREN综合征 

分 类 号：R781.7[医药卫生—口腔医学]