人原发性胆囊癌细胞系SGC-996的建立  

Establishing of Human Primary Gallbladder Cancer Cell Line SGC-996

作　　者：杨耀琴[1] 陶惠红[1] 杨虎川[1] 

机构地区：[1]同济大学医学院肿瘤细胞学研究室,上海200092

出　　处：《同济大学学报（医学版）》2003年第6期457-459,共3页Journal of Tongji University(Medical Science)

基　　金：上海市自然科学基金资助项目(01ZB14069)

摘　　要：目的 建立原发性胆囊癌细胞系,对其生物学特性进行初步观察。方法 从1例女性胆囊乳头状腺癌组织取材,分离癌细胞进行体外细胞培养、传代,进行生物学特性观察。结果 该细胞系为典型的上皮型多边形细胞,在含10%新生小牛血清的RPMI1640培养液中生长稳定,增殖旺盛,有重叠生长现象。群体倍增时间约为48 h,分裂指数较高,染色体数目为亚三倍体。无胸腺裸鼠皮下异体接种全部致瘤。电镜观察细胞表面微绒毛丰富,细胞间偶见桥粒等细胞连接。未见病毒颗粒、支原体及细茵。结论 该细胞符合一般体外培养细胞系标准,定名为SGC-996,可作为人胆囊癌研究的模型。Objective To establish human primary gallbladder cancer cell line SGC-996 and observe its cytobiological characteristics. Methods The gallbladder cancer cells were isolated from the primary mastoid adenocarcinoma of the gallbladder obtained from a 61 year-old female patient. The cancer cells were cultured in Eagle and RPMI 1640 media with fe-tal bovine serum. Results This cell line could grow steadily in RPMI1640 with 10% fetal bovine serum and proliferate rapidly. The cells were epithelial-like and had large nuclei with one or more nucleoli. The cells could form multi-layered colonies. Double Time of cell proliferation was about 48 h. Mitotic index was high. Chromosome frequency distribution be-longed to hypotriploidy. All athymic rude mice could form the transplant tumors in the test of subcutaneous inoculation. A plentiful of microvilli and fewer cell junctions such as desmosomes were observed by transmission electron microscopy. Virus granule,mycoplasma and bacteria were negative. Conclusion SGC-996 cells accorded with the general characteristics of the cell line in vitro.

关 键 词：胆囊肿瘤 细胞系 细胞培养 人 

分 类 号：R737[医药卫生—肿瘤]