建立成骨与破骨细胞共育体系的一种方法  

A Method for Development of a Co-culture System:Osteoblast and Osteoclast Interaction

作　　者：史风雷[1] 王海彬[1] 杨勇[1] 樊粤光[1] 袁浩[1] 

机构地区：[1]广州中医药大学第一附属医院髋关节中心,510405

出　　处：《中国矫形外科杂志》2003年第22期1550-1552,共3页Orthopedic Journal of China

基　　金：中国博士后基金(200031);国家自然基金(30100244);广东省自然基金(020778)

摘　　要：目的:建立成骨细胞和破骨细胞共育的体外模型,为研究骨生理和病理提供新的方法。方法:从胎鼠和4周鼠中分离成骨和破骨细胞,并按照10:1、100:1的比例混合培养,应用碱性磷酸酶(AKP)、抗酒石酸(TRAP)、甲苯胺蓝染色法,鉴定成骨及破骨细胞,并测量碱性磷酸酶的含量,从而建立一个较稳定的共育体系。结果:破骨细胞和成骨细胞达到了相互接触培养,且随着破骨细胞数量的不同,碱性磷酸酶的含量会有不同的变化。结论:该模型可用骨形成和吸收代谢的相关研究。Objective:To establish cocultured model of osteoblast and osteoclast interaction in vitro, according with that in vivo,and to provide a new method for studing physiology and pathology of bone. Methods:The authors isolated osteobalsts from calvarium of fetal rat, and osteoclasts from 4-week-rat marrow of femur and tibiae bone, and cocultred by 10:1,100:1. Cells were stained with alkaline phosphatase( AKP), tartrate-resistant acid phosphatase (TRAP) ,Toluidine Blue technique, the concentration of ALP were examined. Rasult: It was a stable modle of osteoblast and osteoclast coculturing. The AKP were changed with the quantities of osteoclasts. Conclusions: The model can apply in study of formation and absorption of bone metabolism.

关 键 词：成骨细胞 破骨细胞 共育体系 细胞培养 碱性磷酸酶 骨形成 骨吸收代谢 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]