人脑胶质瘤mRNAs的分离纯化及其cDNAs合成  

Purification of mRNAs from Human Glioma and Synthesis of its cDNAs

作　　者：覃文新[1] 王尧[1] 左剑玲[1] 陈桂林[1] 李斌[1] 韦永新 杜子威[1] 赵涵芳[2] 章有章[2] 程枫[2] 陈诗书[2] 

机构地区：[1]苏州医学院脑神经研究室 [2]上海第二医科大学生物化学教研室分子生物学实验室

出　　处：《苏州医学院学报》1992年第2期87-91,168,共5页Acta Academiae Medicinae Suzhou

基　　金：国家自然科学基金

摘　　要：采用异硫氰酸胍/氯化铯超离心法,从人脑恶性胶质瘤手术标本中抽提总 RNA;经两轮Oligo(dT)—cellulose 亲和层析纯化得到 mRNAs。Northern 转移印迹分析表明:mRNAs 无降解,完整性好。以此为模板,经反转录,合成得到双链 cDNAs 分子。cDNA 第1条链的反转录效率为36.4%,第2条链的合成效率为80.8%。This paper reports the extraction of total RNA from two operative specimens of human glioma byguanidinium thiocyanate/cesium chloride ultracentrifugal method.mRNAs were purified by two cyclesof affinity chromatography on oligo(dT)-cellulose.Northern blotting analysis showed that themRNAs were undergraded and its integrity was satisfactory.The double-stranded cDNAs weresynthesized by reverse transcription using this mRNAs as template.The reverse transcription efficiencyof first-strand cDNA was 36.4%.The synthesis efficiency of second-strand cDNA was 80.8%.

关 键 词：互补DNA 脑肿瘤 神经胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]