吐温-80对野葛毛状根生长及异黄酮含量的影响  

Effects of Tween-80 on the Growth and Content of Isoflavones in the Pueraria lobata Hairy Roots

作　　者：于树宏[1] 范庆书[1] 李玲[2] 潘瑞炽[2] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]华南师范大学生命科学学院,广东广州510631

出　　处：《生物技术》2003年第6期41-43,共3页Biotechnology

基　　金：教育部"资助优秀年轻教师基金"项目;广东省科技攻关资助项目 (S990 4 0 1 )

摘　　要：将不同浓度的吐温 - 80添加到野葛毛状根悬浮培养液中 ,研究在一定的作用时间内其对毛状根生长及次生代谢物合成与分泌的影响。结果表明 ,采用 2 %浓度处理较为适宜 ,不仅可以提高毛状根内葛根素的含量 ,而且有利于培养液中葛根素、大豆甙元及总异黄酮的积累 ,与对照相比 ,其含量可分别提高 2 4 .2 %、5 0 %和 4 6 .7%。在该浓度下连续处理毛状根 72h后 ,发现毛状根仍生长旺盛 ,其生长量已是对照的 1.5倍。但不同时间的连续处理对毛状根及培养液中几种异黄酮物质的积累与释放作用不同 ,其中以处理 4 8h最有利于培养液中总异黄酮的累积 ,其含量是毛状根中的 38倍。This article studied the effects of Tween-80 on the growth of hairy roots and the synthesis and release of second substances,which was added into the Pueraria lobata hairy roots suspension culture medium with different concentrations. The results indicated that the supplement of 2% Tween-80 was suitable,because it not only increased the content of puerarin in the hairy roots, but obviously promoted the excretion of puerarin,daidzein and total isoflavones in the medium . Contrast to the control,the contents of every substances in the medium could be raised 24.2%,50% and 46.7%,respectively. When the hairy roots were treated continuously with 2% Tween-80 for 72h,they still grew well and the fresh weight increased 1.5-fold than the control. But the effects were different on the accumulation and release of several isoflavones in the hairy roots and the culture medium. After the treatment of 48h, the content of total isoflavones in the medium was 38 times than that in the hairy roots.

关 键 词：吐温-80 野葛 毛状根 生长 异黄酮 

分 类 号：Q946.8[生物学—植物学] S632.9[农业科学—蔬菜学]