乳糖作为棉铃虫颗粒体病毒增效蛋白表达诱导剂的可行性研究  

EVALUATION OF THE FEASIBILITY OF USING LACTOSE AS THE INDUCER IN PRODUCING THE VIRAL ENHANCING FACTOR FROM HELICOVERPA ARMIGERA GRANULOSIS VIRUS IN ESCHERICHIA COLI BL21DE3

作　　者：董琳茜[1] 张克勤[1] 邱并生[2] 

机构地区：[1]云南大学生物资源保护与利用重点实验室,昆明650091 [2]中国科学院微生物研究所分子病毒学开放实验室,北京100080

出　　处：《微生物学通报》2003年第6期5-9,共5页Microbiology China

基　　金：国家发展计划委员会高技术产业化推进项目(No.1999-1699)

摘　　要：乳糖不仅可以诱导棉铃虫颗粒体病毒增效蛋白表达,而且表达量与IPTG(异丙基-β-D-硫代半乳糖苷)作为诱导剂时相比,并不低于后者,这样大规模生产棉龄虫颗粒体病毒增效蛋白的成本就可能被降低.乳糖的最适浓度经优化为2.2%～2.5%(W/V).对乳糖的不同除菌处理表明:0.70×105Pa 15min蒸汽灭菌的乳糖可以诱导此增效蛋白表达,这种处理比滤膜过滤除菌更为方便和经济,因此就为棉铃虫颗粒体病毒增效蛋白的生产提供了经济实用的前景.Lactose was shown to no less competent than Isopropyl-β-D-thiogalactoside (IPTG) in inducing the expression of the ENHANCIN coding gene from Helicoverpa armigera granulosis virus in Eswcherichia coli BL21 (DE3) regulated by a T7 promoter, since the lactose induction could lead to an ENHANCIN band no smaller than the one in IPTG induction on the SDS-PAGE gel. This would decrease the cost of the large-scale ENHANCIN production. The lactose concentration was optimized at 2.2% - 2.5% (w/v) . Different treatments on the lactose sterilization showed that lactose steam- sterilized in 116. 5℃ for 15min could lead to the ENHANCIN production. The convenience and the relatively low cost in its' operation could further decrease the cost of the ENHANCIN production.

关 键 词：IPTG 乳糖 棉铃虫颗粒体病毒增效蛋白 T7启动子 LAC UV5启动子 

分 类 号：S482.9[农业科学—农药学]