微生物酶法合成L-半胱氨酸和L-胱氨酸  

MICROBIAL ENZYME CONVERSION OF L-CYSTEBSE AND L-CYSTINE

作　　者：刘忠[1] 杨文博[1] 白钢[1] 田旺[1] 金永杰[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《微生物学通报》2003年第6期16-21,共6页Microbiology China

摘　　要：从土壤中分离到一株假单胞菌Pseudomonas sp.TS1138菌株,其胞内含有DL-2-氨基-△2-噻唑啉-4-羧酸(DL-2-Amino-△2-Thiazoline-4-Carboxylic Acid,缩写为DL-ATC)水解酶,以培养16h的细胞为酶源,可转化DL-ATC合成L-半胱氨酸.该菌株生长及产酶的最佳碳、氮源为葡萄糖和尿素,DL-ATC对酶的产生具有诱导作用.酶促反应后的产物经薄层层析、旋光度法和高效液相色谱鉴定为L-半胱氨酸.Pseudomonas sp. TS1138 isolated from soil samples was able to form L-cysteine from DL-2-Amino-△2-Thia-zoline-4-Carboxylic Acid (DL-ATC) after cultured 16 hours . The optimum carbon and nitrogen soruces of strain growth and enzyme formation are glucose and urea. This enzyme was induced by DL-ATC. The product was identified to be L-Cysteine based on thin layer chromatography, optical rotation and HPLC studies.

关 键 词：L-半胱氨酸 L-胱氨酸 酶法转化 假单胞菌 

分 类 号：Q939.9[生物学—微生物学]