浙江榨菜病毒病病原鉴定  

PATHOGEN IDENTIFICATION OF MOSAIC VIRUS DISEASE OF BRASSICA JUNCEA COSS IN ZHEJIANG

作　　者：施曼玲[1] 

机构地区：[1]杭州师范学院生命科学学院,杭州310012

出　　处：《微生物学通报》2003年第6期51-55,共5页Microbiology China

基　　金：浙江省自然科学基金(No.301007)

摘　　要：从浙江桐乡和海宁的花叶或叶片皱缩、植株矮化的榨菜(Brassica juncea Coss)上分离毒源,摩擦接种5种鉴别寄主,在鉴别寄主上都出现不同症状;间接酶联免疫吸附法(In-direct ELISA)检测,芜菁花叶病毒(Turnip mosaic virus,TuMV)的检出率为87.5％,黄瓜花叶病毒(Cucumber mosaic virus,CMV)为73.08％,两者复合侵染率为65.38％,所有的样本都没有检测到烟草花叶病毒(Tobacco mosaic virus,TMV);IC-RT-PCR扩增,分别得到约0.85kb和0.7kb的两条条带,与预期的TuMV和CMV的CP基因大小吻合。CP基因序列测定也表明:所克隆产物是属于TuMV和CMV的CP基因。根据以上试验结果判断:浙江榨菜病毒病主要是由TuMV、CMV以及TuMV和CMV复合侵染引起。The virus isolates were collected from Brassica juncea Coss whose symptom was mosaic, stunt and distorted in Tongxiang and Haining, in Zhejiang province. The isolates could infect five identification hosts and produced different symptoms. The results of indirect ELISA test indicated that the specimens infected by Turnip mosaic virus (TuMV) accounted for 87.5% of the total . 73.08%and 65.48% of the total specimens were infected by Cucumber Mosaic Virus (CMV) and TuMV + CMV complex , respectively. All specimens didn' t infected by Tobacco mosaic virus (TMV) . There were 0. 7kb 和 0.85kb special stripe in 1 % agrose gel electrophoresis after immunocapture polymerase chain reaction (IC-RT-PCR) . All tests results indicated that TuMV, CMV and TuMV + CMV complex were the predominant causal viruses infecting Brassica juncea Coss in Zhejiang province.

关 键 词：榨菜 芜菁花叶病毒 黄瓜花叶病毒 病毒病 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]