家兔消化道5-羟色胺免疫活性细胞的免疫组织化学研究  

Immunohistochemistry Study of 5-HT Positive Immunoreactive Endocrine Cells in Digestive Tract of Oryctolagus cunieulus Rabbits

作　　者：李淑兰[1] 陈洪岩[2] 

机构地区：[1]哈尔滨师范大学生命与环境科学学院生物系,哈尔滨150080 [2]中国农业科学院哈尔滨兽医研究所

出　　处：《中国比较医学杂志》2003年第6期346-349,共4页Chinese Journal of Comparative Medicine

基　　金：蔡火石生物科学发展基金资助

摘　　要：的　通过免疫组织化学染色了解 5_羟色胺 (5_HT)免疫活性阳性细胞在家兔消化道内的分布位置及其形态。方法　取家兔消化道 ,冲洗、固定、石蜡切片 ,用ABC(avidin biotin peroxidasecomplex)免疫组织化学法检测 5 HT阳性细胞。结果　食管和胃贲门部呈阴性反应 ,其他部位均有 5 HT细胞分布 ,分布的密度近似呈字母“M”形。 5 HT细胞形态以圆形或椭圆形为主 ,只有小肠段 5 HT细胞呈梭形具有突起 ,直肠部 5 HT细胞呈锥体形。 5 HT细胞多分布于粘膜上皮或腺泡上 ,但直肠部的 5 HT细胞位于固有膜内。结论　家兔的食性、消化特点对 5 HT细胞分布型形成有一定影响 ,5 HT细胞形态与其内、外分泌功能是相适应的。Objective To study the distribution and morphology of 5-HT pisitive immunoreactive endocrine cells in the digestive tract of %Oryctolagus cunieulus% rabbits by the method of immunhistochemistry technique. Methods The method of the ABC immunhistochemistry technique was performed. Results There was negative reaction in esophagus and cardiac part, and positive reaction in the other parts. The cells' distribution of density was as !M'. The shape of 5-HT positive immunoreactive endocrine cells was round and ellipse mainly, but shuttle with process in small intestine part and tapering in rectum. The cells were found in between epithelial cells and between gastric glands, but in the lamina propria in rectum. Conclusion The feeding habit and digestive character of %Oryctolagus cunieulus% rabbits affect the distribution of 5-HT positive immunoreactive endocrine cells in some extent, the morphology of 5-HT positive immunoreactive endocrine cells are conformable with the endocrine and exocrine functions.;

关 键 词：家兔 消化道 5-羟色胺 免疫活性细胞 免疫组织化学 5-HT 细胞形态学 

分 类 号：R57[医药卫生—消化系统] R392[医药卫生—内科学]