大鼠实验性急性胰腺炎动物模型的建立  

Establishment of Model of Experimental Acute Pancreatitis in Rats

作　　者：李涛[1] 王海燕[1] 刘丽玲[2] 张新臣[3] 郭守利[1] 王海霞[1] 林彦[1] 

机构地区：[1]哈尔滨医科大学附属第二医院动物实验中心,哈尔滨150086 [2]中国农业科学院哈尔滨兽医研究所动物流感中心,哈尔滨150001 [3]哈尔滨医科大学附属第二医院,哈尔滨150086

出　　处：《中国比较医学杂志》2003年第6期358-361,共4页Chinese Journal of Comparative Medicine

基　　金：哈尔滨医科大学附属二院科研基金资助 ( 2 0 0 1-2 8)

摘　　要：目的　利用手术方法建立大鼠实验性急性胰腺炎动物模型。方法　 5 6只Wistar大鼠 ,随机分为 3组 ,实验组 :结扎胆总管末端 ;对照组 :按Nevalainen法 ,结扎胆总管开口远、近各 1cm的十二指肠 ;正常组 :正常Wist ar大鼠。实验组与对照组分别于术后第 10、2 4、4 8h采血测定血清淀粉酶、血清钙及血糖 ,观察胰腺大体病理变化并作组织学检查。结果　实验组及对照组大鼠的血清淀粉酶显著高于正常组 ,血清钙显著低于正常组 ,而血糖差异不显著。结论　经生化分析及组织学检查提示急性胰腺炎 (AP)动物模型建立。Objective To establish an model of experimental acute pancreatitis(AP) in rats by operational method.Methods Wistar rats were divided randomly into three groups.The end of bile duct was ligated in the test group;in the control group placket of duodenum was ligated one centimeter far and near to the bile duct;the normal group as a control:the normal Wistar rats.Test and control groups rats were phlebotomized at 10,24,48h respectively,for detection of the amylase concentration,calcium and blood sugar.At the same time,the pathological change and the histological change were examined.Results The level of amylase concentration of test and control groups increased significantly,and higher than the normal group as a control;the level of calcium reduced significantly,but blood sugar had no remarkable change.Conclusion Biochemical analysis and histological examination demonstrated that the AP model was successfully constructed.;

关 键 词：大鼠 急性胰腺炎 动物模型 手术 AP 病理学 

分 类 号：R576[医药卫生—消化系统] R-332[医药卫生—内科学]