应用四色荧光分析技术测定造血干细胞移植后患者CD45RA^+细胞的变化  

Changes of peripheral blood CD45RA^+ cells after hematopoietic stem cell transplantation:evaluation with four-color flow cytometry

作　　者：阴继霞[1] 孙竞[1] 易正山[1] 周淑芸[1] 

机构地区：[1]第一军医大学南方医院血液科,广东广州510515

出　　处：《第一军医大学学报》2003年第12期1286-1289,共4页Journal of First Military Medical University

基　　金：广东省自然科学基金(010584)~~

摘　　要：目的探讨流式细胞技术测定造血干细胞移植(HSCT)后患者外周血中初始T细胞CD45RA+的变化与临床意义。方法收集来自南方医院的49例造血干细胞移植病人的移植前、移植之后15、30、90和180 d不同时间点的90份肝素抗凝外周血标本。应用流式细胞技术测定外周血CD4+CD45RA+/CD8+CD45RA+细胞数的动态变化。结果在49名造血干细胞移植患者外周血标本中选择90份样本进行检测,移植后30 d,CD8+CD45RA+细胞即能恢复到正常水平,而CD4+CD45RA+细胞产量半年之内仍低于正常并持续缓慢增长。经HSCT患者的胸腺再生输出功能明显低于自体干细胞移植患者(P<0.01)。异体外周血干细胞移植患者的胸腺恢复能力显著比异体骨髓移植患者更快(P<0.05)。结论通过流式细胞仪检测患者外周血中CD4+CD45RA+/ CD8+CD45RA+细胞的变化,可以相对定量地检测患者的胸腺再生输出功能,并通过测量新生T细胞的合成来监视胸腺功能在免疫重建中的作用,为临床干细胞移植治疗提供有力的数据。Objective To investigate the alteration of peripheral blood naive T cells in adult patients after hematopoietic stemcell transplantation (HSCT). Methods Peripheral blood samples were collected from 49 patients requiring stem cell transplantationbefore and 15, 30, 90, and 180 d after transplantation, respectively. Dynamic changes of CD4+CD45RA+/CD8+CD45RA+ cellcounting were measured using flow cytometry. Results Flow cytometry results of 90 samples showed that CD8+CD45RA+cells recovered to normal level 30 d after transplantation, whereas CD4+CD45RA+ cells remained low and kept a slow pace ofgradualincreaseoverthe 6monthsfollowingthetransplantation.Morerapidrecoveryofthetransportandregenerationfunctionofthethymuscellswasachievedinpatientsreceivinghematopoieticstemcellautotransplantthaninthosewithallotransplant. Conclusion Measurement of the changes of CD4+CD45RA+/CD8+CD45RA+ cell ratio using flow cytometrymay provide insights into the molecular picture of the thymus regeneration function in association with immune reconstructionprocess in patients after stem cell transplantation. The number of newly generated T cells transported from the thymus can be aputative marker of immune reconstruction after transplantation.

关 键 词：四色荧光分析技术 测定 造血干细胞移植 CD45RA^+细胞 外周血 初始T细胞 

分 类 号：R446.6[医药卫生—诊断学] R551[医药卫生—临床医学]