应激对大鼠海马金属硫蛋白亚型表达的影响  

EFFECTS OF RESTRAINT STRESS ON THE EXPR ESSION OF METALLOTHIONEINS IN HIPPOCAMPUS OF RAT

作　　者：陈伟强[1] 程义勇[1] 李树田[1] 王冬兰[1] 洪燕[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《营养学报》2003年第4期370-373,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30 1 0 0 1 49)

摘　　要：目的 :　观察应激对大鼠海马脑区金属硫蛋白亚型表达的影响。方法 :　以束缚为应激原建立心理应激动物模型。将动物随机分为四组 :正常对照组 (Ctr)、束缚 1 w组 (R1 )、束缚 2w组 (R2 )和束缚 4w组 (R4)。取动物脑和肝脏以镉饱和法测定金属硫蛋白含量 ,取海马组织提取总 RNA,以 RT- PCR检测金属硫蛋白亚型 MT- 1 m RNA和 MT- 3m RNA,并取血测定血浆皮质醇和 IL- 6水平。结果 :　束缚应激后 ,大鼠体重增长明显减慢 ,血浆皮质醇、IL- 6水平显著高于Ctr组 ;海马脑区两种亚型金属硫蛋白 m RNA的表达水平升高 ,脑和肝脏金属硫蛋白含量也明显增加。结论 :　束缚应激可影响海马金属硫蛋白的表达 ,使其在蛋白质水平和 m RNA水平均出现明显升高 ,其可能机制是 ,在应激条件下皮质醇和 IL-Objective: This study was conducted to evaluate the effect of restraint stress on the expression of metallothioneins in rat. Methods: The animal model of psychological stress was developed by restraint stress. The rats were randomly assigned to four groups as follows: control group and three stress groups that received restraint stress one, two or four weeks respectively. Results: The body weight of stressed rats decreased obviously, and plasma cortisol and IL-6 were both increased significantly in contrast to those of control group. MT-1 mRNA and MT-3 mRNA in hippocampus were also increased in rats receiving restraint s tress. Conclusion: Restraint stress can increase the expressions of metallothioneins and their mRNAs in rats. The possible mechanism is up-regulation of cortisol and IL-6.

关 键 词：应激 大鼠 海马 金属硫蛋白亚型 表达 皮质醇 束缚应激 

分 类 号：R363[医药卫生—病理学]