白细胞介素-1及其抑制剂对眼眶成纤维细胞增殖和合成透明质酸作用的研究  

Study on Effects of Interleukin-1 and Their Inhibitors for Proliferation and Hyaluronic Acid Synthesis of Cultured Human Orbital Fibroblasts

作　　者：罗清礼[1] 张学进[1] 

机构地区：[1]四川大学华西医院眼科,成都610041

出　　处：《Eye Science》2003年第4期232-235,共4页眼科学报（英文版）

基　　金：国家教委博士点基金(C200057);四川省科技厅基金(01SG046-11)

摘　　要：目的:观察白细胞介素1(IL-1)及白细胞介素1受体拈抗剂(IL-1Ra)对甲状腺相关眼病(TAO)病人及正常人眼眶成纤维细胞(OFs)增殖及合成透明质酸(HA)的作用。 方法:取5例TAO病人眼眶组织与眼外肌进行OFs培养,另取4例无眼部病变的意外死亡者眼眶组织与眼外肌进行OFs培养作为对照。分别在其中加入不同浓度的IL-1、IL-1Ra,用MTT法及放射免疫法观察它们对OFs增殖及分泌HA的作用;然后用不同浓度的IL-1Ra与IL-1共同孵育,观察OFs的增殖及HA合成的变化。 结果:IL-1在5U/ml浓度以上时均能明显刺激OFs的增殖和HA的合成,当IL-1浓度为500U/ml时对TAO病人OFs的刺激作用强丁对正常人OFs。IL-1Ra单独使用时对OFs的增殖及HA合成均没有显著性影响。当IL-1Ra(5、50ng/ml)与IL-1(500U/ml)联合孵育时,能阻断IL-1对OFs的刺激作用。 结论:IL-1能刺激TAO病人与正常人OFs的增殖和HA的合成,而IL-1Ra能阻断其刺激作用,因此IL-1Ra可能是TAO治疗中的一个新途径。眼科学报 2003;19:232-235。Purpose:To observe the proliferation and synthesis of hyaluronic acid (HA) of cultured human orbital fibroblasts (OFs) affected by interleukin-1 (IL-1), and their inhibitors interleukin-1 receptor antagonist(IL-1Ra). Methods:OFs from 5 patients with thyroid-associated ophthahnopathy (TAO) and 4 normal subjects were cultured in vitro. IL-1, IL-1Ra with different concentrations were put into media respectively, and IL-1 (500 U/ml),IL-1Ra(5, 50 ng/ml) were put into media together. The proliferation and HA production of OFs were measured by MTT and radioimmunological technique. Results:IL-1 (550 500 U/ml) can significantly increase the proliferation of OFs and synthesis of HA (P<0.05), IL-I ( 500 U/ml) stimulate more proliferation of OFs and HA production of OFs from TAO patients than that of OFs from normal subjects, while IL-1Ra alone had no inhibitive effects at any coneentrations.IL1Ra (5,50 ng/ml) can block the proliferation and HA production of OFs when IL-1 with IL-1Ra incubated together. Conclusion:IL-1 can stimulate the proliferation and HA synthesis of OFs. IL-1Ra is an effective inhibitor of proliferation and synthesis of OFs induced by IL-1. IL-1Ra may be useful in the prevention or treatment of TAO. Eye Science 2003;19:232-235.

关 键 词：白细胞介素-1抑制剂 眼眶 成纤维细胞 细胞增殖 透明质酸 甲状腺相关眼病 白细胞介素-1 

分 类 号：R77[医药卫生—眼科]