转化生长因子-β受体Ⅱ在增生性玻璃体视网膜病变增生膜中的表达  

Expression of TGF-β Receptor Ⅱ in Periretinal Membranes of Proliferative Vitreoretinopathy

作　　者：郭长梅[1] 惠延年[1] 马吉献[1] 韩泉洪[1] 阎峰[1] 秦向阳 王建洲[1] 

机构地区：[1]第四军医大学西京医院眼科研究所,陕西西安710032 [2]山西省武警总队医院眼科,山西太原030006

出　　处：《Eye Science》2003年第4期244-247,共4页眼科学报（英文版）

基　　金：国家自然科学基金(39970780)

摘　　要：目的:观察及评估转化生长因子-β受体Ⅱ(TGF-βRⅡ)在增生性玻璃体视网膜病变(PVR)增生膜中的表达及临床意义。 方法:采用免疫组化和原位杂交方法,对13例PVR患者行玻璃体手术增生膜获得的16例进行TGF-βRⅡ的蛋白和mRNA的检测。 结果:免疫组化结果为:9例C2～C3级膜中,染色反应为阳性的有8例,总阳性率为88.9％。7例D1～D3级膜中有6例为阳性,总阳性率为85.7％。阳性细胞多是一类胞体为长圆形,胞核呈圆形或卵圆形的上皮样细胞。统计学分析TGF-βRⅡ标记与膜分级间无相关性(P>0.05)。原位杂交结果与免疫组化基本一致。 结论:PVR发生过程中视网膜色素上皮细胞在生长因子等的刺激下,TGF-βRⅡ表达显著上调,表明了TGF-β参与PVR增生膜的形成。眼科学报 2003;19:244-247。Purpose:To investigate the expression of transforming growth factor-β receptorⅡ (TGF-βR Ⅱ) in periretinal membranes of proliferative vitreoretinopathy (PVR). Methods:Immunohistochemistry and in situ hybridization were used to detect the expression of TGF-βR Ⅱ protein and mRNA in sixteen proliferative membranes of thirteen PVR patients. Results: Immunohistochemically staining showed that membranes labeled negtive '-'、 weak positive '+',positive '2+'and strong positive staining '3+' were respectively noted one、one、three and four membranes in nine C_2～C_3 membranes. Total positive cell rate is 88.9%. Among seven D_1～D_3 membranes, one membrane was labeled '-', three were '+' and another three membranes were labeled '2+'.Total positive cell rate is 85.7%.Positive staining cells were of a type of epithelial-like cells which frequently found pigment in or around them. Relation between TGF-βR Ⅱ expression and membrane grades appeared no correlation ( P>0.05). Results of in situ hybridization were almost consistent with that of immunohistochemistry. Conclusions:Both TGF-βR Ⅱ protein and mRNA are highly expressed and up-regulated in PVR membranes by cytokine stimulation, moreover TGF-β may play important role in the pathogenesis of PVR. Eye Science 2003 ;19:244-247.

关 键 词：转化生长因子-β受体Ⅱ 增生性玻璃体视网膜病变 增生膜 免疫组化 原位杂交 

分 类 号：R774.1[医药卫生—眼科]