人脑梗死后星形细胞活性与细胞凋亡的相关性研究  

The relationship between activity of astrocytes and apoptosis in human infarcted brains

作　　者：戚基萍[1] 王德生[2] 马广玉 原林[1] 吴爱萍[1] 

机构地区：[1]黑龙江省哈尔滨医科大学第一临床医学院病理科,黑龙江哈尔滨150001 [2]黑龙江省哈尔滨医科大学第一临床医学院神经科,黑龙江哈尔滨150001 [3]黑龙江省牡丹江市先锋医院神经科,黑龙江牡丹江157000

出　　处：《中风与神经疾病杂志》2003年第6期484-486,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金项目 ( 30 2 70 4 80 );黑龙江省自然科学基金项目 ( D0 2 0 3);黑龙江省自然科学基金项目 ( D0 1 46 )

摘　　要：目的　研究人脑梗死后星形胶质细胞活性及凋亡的关系。方法　应用 10例人脑梗死后的尸检全脑标本 ,常规 HE和免疫组织化学方法检测胶质纤维酸性蛋白 (GFAP)、凋亡相关蛋白 (Bcl-2 ) ,进行定性和定量分析。结果人脑梗死后以梗死灶为中心 ,按照组织损伤程度由内到外分为 4个区 (0～ 3区 ) ,其中 2区为半暗带区 ,GFAP在 0区和 1区表达很少 ,在 2区和 3区随缺血时间延长表达迅速持续升高 ;Bcl-2在 0区和 1区表达很少 ,在 2区和3区表达显著。结论　人脑梗死后在半暗带区 2 4h前 GFAP和 Bcl-2表达呈正相关 ;2Objective To investigate the relationship between activit y of astrocytes and apoptosis in human infarcted brains.Methods W e used glial fibrillary acidic protein (GFAP) and anti-apoptotic protein (Bcl- 2) as markers to define changes of cells in post-mortem specimens from ten pati ents by HE and immunohistochemistry.Results Each section of the infarcted brain was divided into four areas (the area 0～3),and the area 2 was known as the ischemic penumbra.The expression of both GFAP and Bcl-2 was lost i n the area 0 and 1,but preserved in the area 2 and 3.GFAP selectively increased in the area 2 and 3 in a time-dependent manner.Conclusion Ther e is relationship between activity of astrocytes and apoptosis that occurred aft er ischemia.

关 键 词：脑梗死 星形细胞活性 细胞凋亡 相关性研究 

分 类 号：R743.33[医药卫生—神经病学与精神病学]