流行性腮腺炎病毒减毒株S_(79)在几株肿瘤细胞和正常细胞中增殖的比较研究  

Comparative study on mumps virus S_(79) proliferating in tumor and normal cell lines

作　　者：严银芳[1] 陈晓[1] 杨小清[2] 闫远芳 

机构地区：[1]武汉大学医学院病毒学研究所,武汉430071 [2]湖北省医学科学院 [3]武汉市铁道第一中

出　　处：《肿瘤》2003年第6期475-478,共4页Tumor

基　　金：国家重点基础研究发展计划项目 (编号 :G19980 5 12 0 9)

摘　　要：目的　比较HeLa、SPC A1、ACHN、HT10 80 4株肿瘤细胞和wish、HELHES、HEK 4株正常人二倍体细胞对MuVS79病毒的敏感性和病毒对细胞的不同影响。方法　细胞感染病毒后用CPE实验和血球吸附实验研究病毒的复制情况 ,用空斑实验测定细胞裂解液的PFU ;用MTT实验 ,活细胞计数 ,流式细胞仪实验 ,TUNEL染色等研究MuVS79对细胞的影响。结果　MuVS79在 4株肿瘤细胞中的增殖能力明显 >4株正常人二倍体细胞 ,敏感顺序为ACHN >HeLa >HT10 80 >SPC A1>HEK>wish >HEL ,HES。染毒后 ,肿瘤细胞的生长速度明显减慢 ,用MTT实验测得HeLa、SPC A1、ACHN、HT10 80、HEK、wishHEL和HES的细胞生长抑制率分别为 38.2 % 33.0 % 4 1.2 % 35 .6 % 7.2 % 5 .6 % 1.2 %和 1.4 %。感染MuVS79后可引起肿瘤细胞G1期细胞阻滞 ,与未染毒对照细胞比较凋亡发生率明显增高 ;而 4株正常人二倍体细胞凋亡发生率未见明显变化。结论　MuVS79具有选择地在肿瘤细胞中增殖和引起肿瘤细胞凋亡的能力。Objective To compare the characteristics of four tumor cell lines (HeLa,SPC-Al, ACHN, HT1080) with that of four human normal diploid cell lines (wish, HEL,HEK, HES) after they were infected by mumps virus S 79(MuV S 79)Methods The proliferous abilities of MuVS 79in tumor and normal cell lines were studied by CPE, plaque and hemagglutination experiments. The characteristics of cells infected by MuVS 79were assayed by MTT, living cell count, flow cyto meter, TUNEL dyeing experiments. Results The proliferous abilities of MuVS 79 in four tumor cell lines were obivously higher than those in four normal cell lines. The susceptible sequences were ACHN>HeLa>HT1080>SPC-A1>HEK>wish>HEL, HES. The growth rates of tumor cells were slowed down after infected by MuVS 79. The growth inhibition ratio of HeLa, SPC-A1, ACHN, HT1080, HEK,wish, HEL and HES lines by MTT experiments were 38.2%, 33.0%,41.2%,35.6%, 7.2%, 5.6%,1.2% and -1.4% respectively. The infection by MuVS 79 could cause G 1 cells retardation in four kinds of tumor cells. The apoptosis rate of tumor cells increased obviously in compare with the untreated control cells, and that of normal cells showed no change. Conclusion MuVS 79 may replicate selectively in cancer cells and may cause the apoptosis of cancer cells.

关 键 词：流行性腮腺炎 病毒减毒株S79 肿瘤细胞 增殖 比较研究 

分 类 号：R73-362[医药卫生—肿瘤]