检测CK19基因对诊断非小细胞肺癌外周血微转移的意义  

Significance of Diagnosing Micrometastasis of CK19mRNA in Peri-pheral Blood of Non-small Cell Lung Cancer

作　　者：丁斐嘉 邵群慧 张鸣号 马胜超[3,4] 朱伯娜 包银萍 王银 

机构地区：[1]宁夏医科大学基础医学院神经生物学系,银川750004 [2]宁夏中卫市人民医院心内科,中卫755000 [3]宁夏心脑血管疾病重点实验室,银川750004 [4]宁夏医科大学基础医学院病理生理学系,银川750004

出　　处：《宁夏医科大学学报》2014年第9期962-965,940,共5页Journal of Ningxia Medical University

基　　金：宁夏回族自治区大学生科技创新项目(2012年)

摘　　要：目的探讨CK19(cytokeratin19,CK19)基因表达在非小细胞肺癌(non-small cell lung cancer,NSCLC)外周血微转移过程中的意义。方法采用real-time PCR检测NSCLC、肺良性病(benign lung disease,BLD)患者和健康人外周血中CK19基因的表达及其与NSCLC外周血微转移的关系。结果 NSCLC微转移患者的CK19 mRNA的表达水平明显高于BLD患者和健康人(P<0.05),其中与健康组比较CK19 mRNA表达水平上升了143%,与BLD组比较CK19 mRNA表达水平上升了53%。结论 CK19基因可作为检测非小细胞肺癌外周血微转移的重要指标。Objective To explore the meaning of CK19( cytokeratin19,CK19) mRNA expression in peripheral blood of non-small cell lung cancer( NSCLC) micrometastases. Methods The expressions of peripheral blood CK19 mRNA and the relationship with peripheral blood of NSCLC micrometastases were analyzed by real-time polymerase chain reaction( real-time PCR) for NSCLC patients,patients with benign lung disease( BLD) and healthy volunteers. Results The positive expression rate of CK19 in NSCLC group was statistically higher than those in BLD patients and healthy volunteers( P < 0. 05). Comparing with healthy volunteers and BLD patients groups,the expression of CK19 mRNA in NSCLC group respectively increased by 143% and51%,respectively. Conclusion CK19 mRNA in peripheral blood can be used as a valuable biomarker for diagnosis of NSCLC micrometastasis.

关 键 词：CK19MRNA 非小细胞肺癌 微转移 

分 类 号：R734.2[医药卫生—肿瘤]