Effect of Muscone on Neuronal Glutamate Transporter EAAC1 Expression in Rats with Acute Cerebral Ischemia  

作　　者：梁辉 陈虎 高颖 蔡定芳 

机构地区：[1]Department of Integrated TCM and Western Medicine, Huashan Hospital, Fudan Uni versity

出　　处：《Chinese Journal of Integrated Traditional and Western Medicine》2003年第4期285-288,共4页中国中西医结合杂志（英文版）

基　　金：This study was supported by the fund of "Hundred Persons Project" of Shan ghai Public Health Bureau (No. 97BR016)

摘　　要：Objective: To investigate muscone's neuroprotective action and it s mechanism in rats with acute cerebral ischemic injury. Methods: Rat focal ischemic model of middle cerebral artery occlusion(MCAO) was establi shed by thread inserting method. TTC staining, RT PCR and Western blot analys is were used to study the effects of muscone on infracted volume, EAAC1mRNA and protein levels.Results: Infarcted volume in the muscone treated gr oup was significantly lower than that in the model control group. Compared with normal group, EAAC1 mRNA level increased in the model control group at 6 and 24 hrs, and protein level increased at 24 hrs after modelling, muscone could reduc e the increased expression in model rats. Conclusion: Muscone po ssesses the action of neuroprotection, its mechanism may be related to reduction of EAAC1 and protein expressions.Objective: To investigate muscone's neuroprotective action and it s mechanism in rats with acute cerebral ischemic injury. Methods: Rat focal ischemic model of middle cerebral artery occlusion(MCAO) was establi shed by thread inserting method. TTC staining, RT PCR and Western blot analys is were used to study the effects of muscone on infracted volume, EAAC1mRNA and protein levels.Results: Infarcted volume in the muscone treated gr oup was significantly lower than that in the model control group. Compared with normal group, EAAC1 mRNA level increased in the model control group at 6 and 24 hrs, and protein level increased at 24 hrs after modelling, muscone could reduc e the increased expression in model rats. Conclusion: Muscone po ssesses the action of neuroprotection, its mechanism may be related to reduction of EAAC1 and protein expressions.

关 键 词：muscone cerebral ischemia  neuronal glu tamate transporter NEUROPROTECTION 

分 类 号：R651.1[医药卫生—外科学]