尼膜同对戊四氮致痫大鼠海马神经细胞凋亡和p^(53)蛋白表达的影响  

Effects of Nimotopine on neuronal apoptosis and p^(53) protein expression in hippocampal area of the rats with pentylenetetrazol-induced epilepsy

作　　者：程桂玲[1] 迟兆富[2] 赵玉英[2] 杜萍 

机构地区：[1]山东省交通医院神经内科,济南250031 [2]山东大学齐鲁医院神经内科 [3]临朐县中医院

出　　处：《陕西医学杂志》2004年第1期6-8,29,共4页Shaanxi Medical Journal

基　　金：山东省科技项目

摘　　要：目的 :探讨尼膜同对癫痫发作大鼠海马神经细胞凋亡与 p53蛋白表达的影响。方法 :采用戊四氮致痫大鼠模型 ,以原位末端标记 ( TUNEL)法检测凋亡细胞 ;免疫组化法检测 p53蛋白。观察大鼠癫痫发作后 48h其海马 CA1区神经细胞凋亡和 p53蛋白表达变化及尼膜同对它们的影响。结果 :戊四氮致痫后 ,大鼠海马 CA1区凋亡细胞和 p53蛋白表达均明显增加 ,p53蛋白表达与细胞凋亡呈正相关 ( r=0 .846,P<0 .0 0 1 ) ;尼膜同干预后 ,凋亡细胞数及 p53蛋白表达均显著性降低 ( P<0 .0 0 1 )。结论 :尼膜同对癫痫所致神经细胞凋亡有抑制作用 ,其抑制凋亡的分子机制可能是通过抑制Objective:To study the effects of Nimotopine (NM) on the neuronal apoptosis and p 53 protein expression in the hippocampal area of the rats with pentylenetetrazol(PTZ)-induced epilepsy. Methods:The model of PTZ- induced epilepsy was used,TUNEL technique and immunohistochemistry method were used to measure the neuronal apoptosis and expression of p 53 protein.After the epilepsy stopped for 48h , effects of NM on the neuronal apoptosis and p 53 protein expression in the hippocampus CA 1 region of the rat were observed. Results:After PTZ- induced epilepsy, apoptotic neurons and p 53 protein expression increased significantly.Correlation analysis showed that neuronal apoptosis had positive correlation with p 53 protein expression(r=0.846,P<0.001). Apoptotic neurons and p 53 protein expression in NM groups were markedly less than those in epilepsy group(P<0.001). Conclusion: NM can inhibit the hippocampal neuronal apoptosis and p 53 protein expression induced by acute seizures, molecular mechanisms of which may be attributed to inhibit p 53 protein expression.

关 键 词：尼膜同 戊四氮 大鼠 海马 神经细胞凋亡 P^53蛋白 癫痫 钙通道阻滞剂 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R972[医药卫生—临床医学]