NGF放射免疫分析及其应用  

Establishment of a Method of NGF Radioimmunoassay and its Primary Application

作　　者：赵小林[1] 

机构地区：[1]第二军医大学神经生物学教研室,200433

出　　处：《放射免疫学杂志》2003年第6期331-333,共3页Journal of Radioimmanology

摘　　要：目的 :建立神经生长因子 (NGF)放射免疫分析 ,并初步探讨其应用价值。方法 :将NGF免疫新西兰家兔制备抗体。建成NGF放射免疫分析法 ,研制成功放免试剂盒。应用本试剂盒测定 81例抑郁症患者血浆和 32例正常人血浆 ,4 0例小鼠颌下腺组织中NGF水平。结果 :灵敏度为 1 0 pg/ml。抗血清特异性 ,与GDNF、EGF、CNTF、β -EP、CCK、GHRH、GH、BNP、Bombesin等均无交叉反应。批内变异系数 <3 85 % (n =1 0 ) ,批间变异系数 <5 1 7% (n =5 )。抑郁症患者血浆NGF浓度为 36 0 0 5± 5 1 89pg/ml,正常为 31 3 5 3±34 0 2 pg/ml。小鼠颌下腺组织中NGF浓度为 2 0 2 0 9± 32 6 1ng/mg。 结论 :NGF放射免疫分析灵敏度高、操作简便 。Objective To develop a method of RIA for measurement of nerve growth factor (NGF) and investigate its primary application. Methods Purified NGF was used to immunize New Zealand rabbits to prepare the antibody against NGF and RIA kits were developed from it. Plasma NGF concentrations of 81 patients with depressive syndrome and 32 controls were determined with this RIA kit. Contents of NGF in 40 mouse submaxiliary glands were also measured. Results The senstivity of the assay was 10pg/ml. The specificity of NGF anti-serum was high and did not react with GDNF、CNTF、EGF、β-EP、CCK、GHRH、GH、BNP、AVP and Bombesin. The intra and interassay CVs were<3 85%(n=10) and <5 17%(n=5) respectively. The plasma levels in patients with depressive syndrome were 360 05±51 89pg/ml and the plasma levels in controls were 313 53±34 02pg/ml. The mouse submaxillary glands contents were 202 09±32 61ng/mg. Conclusion RIA of NGF with this kit is highly sensitive, simple and rapid. It is a reliable means for studying the role of NGF in various physiological and pathophysiological states.

关 键 词：神经生长因子 放射免疫分析 抑郁症 抗体 动物实验 

分 类 号：R749.4[医药卫生—神经病学与精神病学]