仿水溶剂促进酶促拆分环氧丙醇的研究  

Enzymatic resolution of glycidol improved by water-mimicking solvent

作　　者：王智[1] 苟小军[2] 于大海[1] 郑良玉[1] 翁樑[1] 张桂荣[1] 曹淑桂[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130023 [2]成都大学生物工程系分子进化工程实验室,成都610106

出　　处：《吉林大学学报（理学版）》2004年第1期126-129,共4页Journal of Jilin University:Science Edition

基　　金：国家自然科学基金(批准号:20272017).

摘　　要：利用脂肪酶在非水介质中对外消旋环氧丙醇进行不对称酯合成反应,重点研究了强极性有机溶剂作为仿水溶剂完全替代反应体系中的微量"必须"水对酶促拆分反应的影响.实验结果表明,乙腈作为仿水溶剂(最佳用量为0.4%)在适量无水硫酸钠(0.04g左右)的配合下,可以有效除去酯合成反应产生的水.在最适反应条件下,制备得到光学纯度约为90%的单一手性环氧丙醇酯.The asymmetric esterification of glycidol catalyzed by lipase was carried out in a nonaqueous medium. The effect of the replacement of water necessary for the function of lipase with water-mimicking solvent on the resolution was investigated. It's found that the water produced from enzymatic esterification could be removed successfully by addition of acetonitrile((0.4%,) volume fraction) and (0.04　g) anhydrous (Na_2SO_4) into the reaction system. Under the optimal conditions, (R-glycidyl) butyrate with about (ee 90%) was prepared.

关 键 词：仿水溶剂 环氧丙醇 脂肪酶 酯合成反应 乙腈 非水酶学 

分 类 号：O624.31[理学—有机化学] O629.8[理学—化学]