吲哚美辛对白血病细胞增殖和细胞衰老的影响  

Effects of Indomethacin on Proliferation and Senescence of Leukemia Cells

作　　者：张毅[1] 焦向英[1] 

机构地区：[1]山西医科大学生理学系,山西太原030001

出　　处：《中国实验血液学杂志》2015年第6期1586-1590,共5页Journal of Experimental Hematology

摘　　要：目的:观察吲哚美辛对白血病细胞体外增殖与细胞衰老的影响,探讨非甾体类抗炎药物对白血病的辅助治疗作用。方法:吲哚美辛30μmol/L处理U266、K562、U937细胞株,连续培养7 d后,以台盼蓝拒染法检测细胞活率,流式细胞术检测细胞周期和凋亡,用细胞衰老检测试剂盒检测各组细胞各时间点(0、4、7 d)的细胞衰老情况,RT-PCR检测增殖抑制基因P21、P27 mRNA的表达水平。结果:药物作用后U266、U937的细胞活率显著降低(P<0.01)。K562细胞活率未发生显著变化。U266、U937细胞均于G2/M期发生不同程度阻滞,K562细胞周期变化不明显。U266、K562、U937的细胞凋亡率升高(P<0.01)。药物处理后,除U937细胞的P27变化不明显外,U266和K562细胞系的P21和P27 mRNA表达水平均显著增高。K562、U937细胞衰老率明显增高(P<0.01)。结论:吲哚美辛对白血病细胞具有抑制作用,但其机制可因白血病细胞的种类不同而异;提示非甾体类抗炎药物可对白血病起辅助治疗作用,但需根据白血病的类型适当选择。Objective:To investigate the effects of indomethacin on proliferation and senescence of leukemia cells and to analyze whether non-steroidal anti-inflammatory drugs have any adjunctively therapeutic effects on leukemia.Methods:Three kinds of leukemia cell lines(U266,K562 and U937) were treated with either indomethacin(final concentration 30 μmol/L) or solvent DMSO(control) for 7 days.Cell viability was determined by trypan blue,cell apoptosis and cell cycle were determined by flow cytometry,mRNA expression of hyperplastic suppression gene P21 and P27 was determined by RT-PCR,and cell senescence rate was assayed at day 0,4,7 after treatment.Results:After treatment with indomethacin,the cell viability in U266 and U937 groups decreased significantly,while it was not changed in K562 group.Cell cycle in U266 and U937 groups was blocked at G_2/M phase,but the blooking effect was not found in K562 cells.The cell apoptotic rate was enhanced in 3 treated groups(P <0.01).The mRNA expression of P21 and P27 was significantly increased after indomethacin treatment in U266 and K562 cell lines except for the P27 mRNA expression of U937.The expression levels of P21 and P27 mRNA in U266 and K562 increased obviously.The cell senescence rate in K562 and U937 group also increased.Conclusion:The indomethacin possesses inhibitory effects on leukemia cells.However,its mechanisms are varied from different types of leukemia cells.It suggests that nonsteroidal anti-inflammatory drugs can play a role in adjuvant therapy for leukemia,while its application would be adjusted according to different types of leukemia.

关 键 词：吲哚美辛 U266细胞 K562细胞 U937细胞 细胞凋亡 细胞衰老 

分 类 号：R733.7[医药卫生—肿瘤]