共焦显微镜在临床诊断真菌性角膜炎中的应用  

Clinical diagnosis of fungal keratitis using confocal microscopy

作　　者：潘飞[1] 张蓓[1] 姚玉峰[1] 裘文亚[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院眼科,杭州310016

出　　处：《中国实用眼科杂志》2004年第1期23-25,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的　评价共焦显微镜在临床诊断真菌性角膜炎中的应用价值。方法　用共焦显微镜对5 2例临床拟诊为真菌性角膜炎的患者进行检查 ,同时行角膜病灶刮片培养 ,对两组结果进行比较。结果　综合病史、临床表现及治疗结果 ,有 45例临床诊断为真菌性角膜炎 ,其中 3 2例角膜刮片培养到真菌 ,阳性率为 71 11% ( 3 2 /4 5 ) ;43例共焦显微镜检查观察到真菌菌丝和 /或孢子 ,阳性率为 95 5 6% ( 4 3 /4 5 )。结论　共焦显微镜可以在活体角膜上直接观察到真菌菌丝 ,是一种快速、有效和无损伤的检查方法 ,对真菌性角膜炎的早期诊断、治疗和研究等具有重要的临床价值。Objective The aim of the current study was to evaluate the value of confocal microscope in clinical diagnosis of fungal keratitis.Methods In vivo confocal microscopy was performed on 52 patients of suspected fungal keratitis,the results were compared with the culture of corneal scrapings.Results 45 patients were clinical diagnosed of fungal keratitis based on history,clinical signs and treatment response,among which 32 patient′s corneal scrapings were cultured positive with fungi(71.11%)and 43 patient′s confocal images showed fungal filaments and/or spores(95.56%).Conclusions Confocal microscopy can observe fungal filaments in vivo condition,it can be a rapid,effective and noninvasive imaging technology,which is clinical valuable in the early diagnosis,treatment and study of fungal keratitis.

关 键 词：共焦显微镜检查 诊断 真菌性角膜炎 角膜刮片 真菌培养 

分 类 号：R772.21[医药卫生—眼科] R519[医药卫生—临床医学]