盐酸米诺环素HPLC的流动相探讨  

Study on Mobile Phase in Assay of Minocycline Hydro Chloride

作　　者：吴宏伟[1] 郭伟斌[1] 苏智阳[1] 邱元素[1] 

机构地区：[1]福建省厦门市药品检验所,厦门361012

出　　处：《中国药品标准》2003年第6期52-54,共3页Drug Standards of China

摘　　要：目的:建立新的HPLC流动相分析方法,使米诺环素与甲基米诺环素达到有效的分离,测定准确的米诺环素含量。方法:流动相为醋酸铵缓冲溶液(pH6.55):乙腈:甲醇=830:185:5,检测波长为280nm,流速为1.0ml/min,进样量为20μl。结果:米诺环素与甲基米诺环素的分离度大于2.3,线性范围在0.2mg/ml～2mg/ml,r=0.9999;回收率为99.22％,95％可信限范围为99.22±0.52％(n=9),RSD=1.14％。Objective: To establish a new analytical method of HPLC to determinate accurately the potency of Minocycline by Changing mobile phase. It can make Minocycline and Methylol Minocycline to achieve an effective separation. Method: Mobile phase is ammonium acetate buffer solution(pH6. 55) : acetonitrile : methanol = 830:185 :5. wavelength was 280nm,flow rate was 1.0ml/min,volume injected was 20μl. Result: Separablity of Minocycline exceed 2. 3,the linear range was 0. 2-2mg/ ml,r = 0. 9999,the recovery was 99. 22%,the 95% creditability range was 99. 22±0. 52% (n = 9) ,RSD= 1. 14%.

关 键 词：盐酸米诺环素 四环素类抗生素 高效液相色谱法 流动相分析 含量测定 

分 类 号：R978.14[医药卫生—药品] R927.2[医药卫生—药学]