Acute effects of huperzine A and tacrine on rat liver  

石杉碱甲和他克林对大鼠肝脏的急性效应(英文)

作　　者：马小超[1] 欣坚 王海学 张婷 屠曾宏 

机构地区：[1]中国科学院上海生命科学院上海药物研究所新药研究国家重点实验室,上海200031 [2]复旦大学药学院药理教研室

出　　处：《Acta Pharmacologica Sinica》2003年第3期247-250,共4页中国药理学报（英文版）

摘　　要：AIM: To observe the acute effects of huperzine A and tacrine on rat liver. METHODS: Changes of liver coefficient,serum biochemistry, and histopathology were detected after single dose. In vitro cytotoxicity was assessed by determining extracellular and intracellular amount of lactate dehydrogenase in cultured hepatocytes. RESULTS:Both huperzine A and tacrine raised liver coefficient and increased serum aspartate aminotransferase and alanine aminotransferase. Tacrine induced liver histopathologic changes. The acute effects of huperzine A on liver could be redressed by atropine, while effects of tacrine on liver could not. Concentration-dependent in vitro cytotoxicity occurred with tacrine, but not with huperzine A. CONCLUSION: The acute effects of huperzine A on rat liver are not related to hepatotoxicity. The acute effects of tacrine on rat liver are related to hepatotoxicity.目的:观察石杉碱甲(HupA)和他克林(TAH)对大鼠肝脏的急性效应。方法:单次给药后检测肝脏系数和血液生化指标,观察组织病理学变化。肝细胞体外培养检测细胞内外乳酸脱氢酶。结果:HupA和TAH均可引起大鼠肝脏系数增加,天冬酸氨基转移酶和丙氨酸氨基转移酶增高。TAH可导致肝脏组织病理学变化。阿托品可纠正HupA对肝脏的急性效应,而不能纠正TAH对肝脏的急性效应。肝细胞体外培养显示TAH可引起剂量相关的细胞毒性,而未见HuDA有肝细胞毒性。结论:HupA对大鼠肝脏的急性效应与肝毒性无关,TAH对大鼠肝脏的急性效应与肝毒性有关。

关 键 词：cholinesterase inhibitors huperzine A TACRINE LIVER acute toxicity test 

分 类 号：R96[医药卫生—药理学]