Cladonia furcata polysaccharide induced apoptosis in human leukemia K562 cells  

分枝石蕊多糖诱导人白血病K562细胞凋亡(英文)

作　　者：林忻[1] 蔡育军[2] 李志孝[2] 刘中立[2] 尹少甫[1] 赵进昌[1] 

机构地区：[1]兰州医学院微生物免疫教研室 [2]兰州大学应用有机化学国家重点实验室,兰州中国730000

出　　处：《Acta Pharmacologica Sinica》2001年第8期716-720,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China,No 39730480

摘　　要：AIM: To study whether Cladonia furcata polysaccharide (CFP-1) might induce apoptosis in human leukemia K562 cells. METHODS: Inhibition of proliferation was measured by MTT assay. Morphological assessment of apoptosis was performed with fluorescence microscope and electron microscope. DNA fragmentation was visualized by agarose gel electrophoresis. The amount of apoptosis cells was measured by flow cytometry. RESULTS: CFP-1 (50-800 mg/L) inhibited K562 cell proliferation in a concentration-dependent manner. After incubation of K562 cells with CFP-1 300 mg/L for 5 d, morphological changes of typical apoptosis were observed and agarose gel electrophoresis of DNA revealed 'ladder' pattern. CONCLUSION: CFP-1 induced apoptosis in human leukemia K562 cells.目的:研究分枝石蕊多糖(CFP-1)是否能诱导K562 细胞凋亡。方法:抑制细胞增殖的测定采用MTT法;用荧光显微镜和透射电镜观察细胞的形态学变化;采用琼脂糖凝胶电泳法观测DNA碎片;用流式细胞仪检测凋亡细胞数。结果:CFP-1(50-800mg/L)明显抑制K562细胞增殖,并且呈浓度依赖性。K562细胞与CFP-1 300mg/L共同培养5d后,观察到典型的凋亡形态变化,电泳呈现梯形条带。结论:CFP-1诱导人白血病K562细胞凋亡。

关 键 词：Cladonia furcata POLYSACCHARIDES apoptosis K562 cells DNA fragmentation ELECTROPHORESIS flow cytometry 

分 类 号：R733.7[医药卫生—肿瘤]