双歧杆菌对实验性大肠癌丝裂素活化蛋白激酶和激活蛋白-1的影响  

Effect of bifidobacteria on MAPK and AP-1 of experimental large bowel carcinoma

作　　者：戴建宜[1] 王立生[1] 杨冬华[2] 朱惠明[3] 潘令嘉 马晓东[4] 张亚历[5] 周殿元[5] 

机构地区：[1]暨南大学医学院第二附属医院深圳市人民医院消化疾病研究所,深圳518020 [2]暨南大学医学院第一附属医院消化科 [3]暨南大学医学院第二附属医院 [4]第一军医大学基础部中心实验室 [5]第一军医大学南方医院全军消化疾病研究所

出　　处：《中华实验外科杂志》2003年第12期1119-1120,共2页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目(994066)

摘　　要：目的 探索青春型双歧杆菌的体内抑瘤机制。方法 以激光共聚焦显微镜检测了大肠癌裸鼠移植瘤细胞外信号调节蛋白激酶(ERK)1/2、c-jun氨基末端激酶(JNK)和p38以及激活蛋白-1(AP-1)的主要成份 c-fos和c-jun的含量。结果 大肠癌裸鼠移植瘤经双歧杆菌处理后,其ERK1/2、JNK、c-fos和c-jun的平均荧光强度均显著低于肿瘤对照组(P<0.01),而p38的平均荧光强度在两组间差异无显著性(P>0.05)。结论 青春型双歧杆菌体内能明显降低大肠癌ERK1/2和JNK的活性以及c-fos和c-jun的表达。Objective To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. Methods The content of extracellular signal regulated protein (ERK) 1/2, C-Jun N-terminal kinase (JNK), p38, c-fos and c-jun of nude mouse transplantation tumors of large bowel carcinoma was detected by using laser scanning confocal microscopy. C-fos and c-jun were the main composition of activator pro- tein 1. Results After the nude mouse transplantation tumors of large bowel carcinoma were treated with bifidobacteria, the average fluorescent intensity of ERK1/2, JNK, c-fos and c-jun was significantly lower than that in tumor control group (P < 0. 01). There was no significant difference in the average flures- cent intensity of p38 between two groups (P > 0. 05). Conclusion Bifidobacteria adolescence could markedly decrease the activity of ERK1/2, JNK and the expression of c-fos, c-jun.

关 键 词：双歧杆菌 实验 大肠癌 丝裂素活化 蛋白激酶 激活蛋白-1 

分 类 号：R735.34[医药卫生—肿瘤] R73-3[医药卫生—临床医学]