胰岛素样生长因子-II对卵巢癌细胞SKOV3中MMP-9表达的调节作用  

Effect of IGF-II stimulation on the expression of matrix metalloproteinase-9 in human ovarian cancer cell line SKOV3

作　　者：吴小华 王利红[2] 宋俊芬[1] 王伟萍[3] 单保恩[1] 

机构地区：[1]河北医科大学第四医院妇产科,石家庄050011 [2]山东大学医学院 [3]北京市普仁医院妇产科

出　　处：《现代妇产科进展》2003年第6期413-415,共3页Progress in Obstetrics and Gynecology

基　　金：河北省自然科学基金资助项目(项目编号 30 1391)

摘　　要：目的 :探讨胰岛素样生长因子 II(IGF II)对卵巢癌细胞SKOV3的基质金属蛋白酶 9(MMP 9)、金属蛋白酶组织抑制剂 1(TIMP 1)表达的影响。方法 :细胞 (1.5× 10 5/ml)培养 4 8h后 ,加入不同浓度的IGF II ,再培养 18h。用RT PCR法测定MMP 9mRNA、TIMP 1mRNA水平 ,用明胶酶谱法半定量测定MMP 9蛋白水平的变化。结果 :IGF II可刺激MMP 9蛋白的分泌。随着IGF II浓度增加 ,诱导作用增强 ;浓度较高时 ,MMP 9的分泌量轻度下降。但IGF II不影响MMP 9和TIMP 1的基因表达水平。结论 :IGF II可刺激卵巢癌细胞SKOV3中MMP 9的分泌 。Objective:To investigate the effect of IGF II on MMP 9 and TIMP 1 expressions in ovarian cancer cell line SKOV3.Methods:SKOV3 cells (1.5×10 5/ml) were cultured for 48 hours and then treated with IGF II at different concentration for additional 18 hours culture.MMP 9 secretion in conditional medium was analyzed by gelatin zymography.MMP 9 and TIMP 1 mRNA were measured by RT PCR.Results:The secretion of MMP 9 in SKOV3 increased after the treatment of IGF II.This stimulation was enhanced with increasing concentration of IGF II.However,treated with higher IGF II concentration (100ng/ml),MMP 9 secretion slightly decreased.Nevertheless,the expressions of MMP 9 and TIMP 1 mRNA had no significant response to IGF II treatment.Conclusion:IGF II may play an important role in the process of invasion and metastasis in ovarian cancer through increasing MMP 9 secretion.

关 键 词：胰岛素样生长因子Ⅱ 卵巢肿瘤 基质金属蛋白酶 金属蛋白酶组织抑制剂-1 聚合酶链反应 RT-PCR 

分 类 号：R737.31[医药卫生—肿瘤]