三氧化二砷对人喉鳞癌细胞株Hep-2增殖抑制和细胞凋亡作用的体外研究  

A study in the Effect of Arsenic Trioxide on Proliferation Inhibition and Cell Apoptosis to Hep-2 in Vitrc

作　　者：张健梅[1] 李玉春[2] 李丽敏[3] 孙河太[1] 李慧军[4] 袁慧筠[4] 张天虹[4] 

机构地区：[1]武警北京总队医院耳鼻咽喉-头颈外科,100027 [2]哈尔滨医科大学附属第一临床医学院耳鼻咽喉-头颈外科,150001 [3]哈尔滨医科大学附属第一临床医学院耳鼻咽喉-,血液内科150001 [4]哈尔滨医科大学附属第一临床医学院耳鼻咽喉-头颈外科内科,150001

出　　处：《中国眼耳鼻喉科杂志》2004年第1期15-17,共3页Chinese Journal of Ophthalmology and Otorhinolaryngology

摘　　要：目的观察三氧化二砷(Arsenic trioxide,As_2O_3)对人喉鳞癌细胞株Hep-2细胞的作用,为晚期喉癌的综合治疗提供实验依据。方法以不同浓度的As_2O_3作用于Hep-2,利用细胞生长曲线、MTF法、瑞氏-吉姆萨(Wrighs-Gimesa)染色,DNA凝胶电泳技术,TUNEL,流式细胞术,观察其细胞增殖抑制、凋亡及细胞周期作用。结果 As_2O_3对Hep-2具有增殖抑制作用,显示时间和剂量效应。在一定剂量和时间范围内,主要引起细胞凋亡,并具有药物浓度依赖性,细胞大部分阻滞在G_2+M期。结论 As_2O_3对Hep-2具有增殖抑制及诱导调亡的双重作用,为喉癌治疗提供了一个具有可行性的设想和依据。Purpose To investigate the effect of arsenic trioxide (As2O3) on Hep-2 cell lines of laryngeal squamous cell carcinoma and provide the experimental basis for the clinical therapy of terminal laryngeal carcinoma. Methods As2O3 at various levels of concentration were used in Hep-2 in vitro. To observe proliferation inhibition, apoptosis and the cell cycle with the growth curve of cell lines, MTT, Wrigh's-Gimesa.DNA agarose gel electrophoresis technique, TUNEL and flow cytometry were used. Results Hep-2 proliferation inhibition was affected by As2O3 and showed the effect of time and dosage. Within a certain time and dosage, apoptotic cells were induced, which depended on As2O3 concentration, Most of the cells were arrested in the G2+M period. Conclusion Arsenic trioxide can induce apoptosis and proliferation inhibition of Hep-2 cell lines of laryngeal squamous cell carcinoma, which can provide a practicable way for the clinical therapy of terminal laryngeal carcinoma.

关 键 词：喉鳞癌 HEP-2细胞 三氧化二砷 细胞增殖 细胞凋亡 

分 类 号：R739.65[医药卫生—肿瘤]