重组内皮抑素对角膜新生血管抑制作用的实验研究  

An Experimental Study of the Inhibitive Effect of Recombinant Endostatin on Corneal Neovascularization

作　　者：袁中芳[1] 魏增涛[1] 牟国营[1] 王晓燕[2] 陶祥臣[1] 李新[1] 王红梅[1] 朱丽贞[1] 

机构地区：[1]济南市中心医院眼科,250013 [2]山东大学基础医学院免疫研究所

出　　处：《中国眼耳鼻喉科杂志》2004年第1期10-12,共3页Chinese Journal of Ophthalmology and Otorhinolaryngology

基　　金：山东省医药卫生科研项目(2001CA2CAA1)

摘　　要：目的探讨重组内皮抑素(Endostatin)对兔角膜新生血管(corneal neovascularization,CNV)形成的影响。方法新西兰白兔18只,随机分为三组,每组6只12眼,采用角膜缝线法制备兔CNV模型,其中两组分别以浓度为10 μg/ml、50μg/ml重组内皮抑素点眼,4次/d,连续19 d,并以生理盐水点眼作对照。CNV生长面积采用计算机图像分析系统进行分析比较。结果10μg/ml、50μg/ml重组内皮抑素实验组自第7d开始CNV平均生长面积明显低于对照组(P<0.05),两实验组CNV面积比较,差异无显著意义(P>0.05)。角膜组织病理学检查,实验组新生血管明显稀少,实验组与对照组炎性细胞成分及浸润程度相似。结论浓度10 μg/ml、50μg/ml重组内皮抑素具有明显抑制角膜新生血管生长的作用,而无毒副作用,为临床防治CNV提供了一种新的途径.Purpose To investigate the effect of recombinant human endostatin on comeal neovascularization (CNV). Methods Eighteen New Zealand white rabbits were induced to neovascularization by stitching cornea. They were randomly divided into 3 groups,6 rabbits each group. Eye drops of recombinant endostatin with different concentrations were used four times daily for 19 days in each group: 10 μg/ml in group 1,50 μg/ml in group 2 and normal saline in group 3 (control) .The CNV area was determined by computer imaging analysis system. Results The mean CNV area of 10 μg/ml and 50 μg/ml was significantly smaller than that of control groups from the seventh day( P < 0.05) . No significant difference was found between group 1 and group 2. Conclusion Recombinant human endostatin has prominent effectiveness on the inhibition of comeal angiogenesis.

关 键 词：角膜新生血管 重组内皮抑素 计算机图象分析系统 组织病理学检查 

分 类 号：R772[医药卫生—眼科]