人乳头状瘤病毒16/18型在口腔疣状癌中的表达及意义  

Expression of human papillomavirus types 16 and 18 in oral verrucous carcinoma

作　　者：邹萍[1] 唐瞻贵[2] 冯德云[2] 沈子华[2] 姚志刚[2] 

机构地区：[1]深圳市中医院口腔科,518033 [2]长沙中南大学湘雅医院

出　　处：《现代口腔医学杂志》2004年第1期5-8,共4页Journal of Modern Stomatology

摘　　要：目的　研究人乳头状瘤病毒 16 /18型在口腔疣状癌中的表达状况 ,探讨其在口腔疣状癌发生发展中的生物学意义。方法　采用SP免疫组化和原位杂交方法分别检测 8例正常口腔粘膜、13例疣状癌、10例高分化鳞状细胞癌、10例低分化鳞癌组织中HPV16 /18E6蛋白和HPV16 /18DNA的表达。结果　①疣状癌HPV16 /18E6蛋白及HPV16 /18DNA阳性表达率均为 6 9.2 % (9/13) ,E6蛋白平均染色强度高于高分化鳞状细胞癌和低分化鳞状细胞癌组 (P <0 .0 5 )。②免疫组化方法检测HPV16 /18E6蛋白与原位杂交方法检测HPV16 /18DNA结果有良好的一致性。结论　HPV16 /18型感染是口腔疣状癌的重要致病因子 ,与高分化鳞状细胞癌、低分化鳞状细胞癌组织相比 ,HPV16 /18型感染与疣状癌的关系更为密切。Objective To investigate the expression of human papillomavirus types 16 and 18 (HPV16/18) in oral verrucous carcinoma (VC) and to reveal their roles in the carcinogoenesis of VC.Methods Streptavidin/peroxidase(SP) immunohisto-chemical technique(IHC) and in situ hybridization(ISH) were used in this study,the expressions of HPV16/18 E6 protein and HPV16/18 DNA in 41 samples,including 8 normal mucosa,13VC,10 well differentiated squamous cell carcinoma(wd SCC),10 poorly differentiated squamous cell carcinoma(pd SCC) were observed.Results ①Both HPV16/18 E6 protein and HPV16/18 DNA were identified in 69.2% (9/13) of VC.The mean intensity of E6 protein in VC was higher than that in wdSCC and pdSCC(P< 0.05 ).②The significant concordence between IHC and ISH was shown.Conclusion HPV16/18 infection is an important epiological agent of VC.The pathogenesis of VC is more closely associated with HPV16/18 infection than wdSCC and pdSCC.

关 键 词：16/18型人乳头状瘤病毒 口腔疣状癌 免疫组化 原位杂交 检测 

分 类 号：R739.81[医药卫生—肿瘤]